LOCUS       sequence-86766-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-ER-5
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             156..836
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcgccacca tgctgctatc cgtgccgttg ctgctcggcc
      121 tcctcggcct ggccgtcgcc gcaccggtcg ccaccatgaa cagcctgatc aaagaaaaca
      181 tgcggatgaa ggtggtgctg gaaggcagcg tgaacggcca ccagttcaag tgcaccggcg
      241 agggcgaggg caacccctac atgggcaccc agaccatgcg gatcaaagtg atcgagggcg
      301 gacctctgcc cttcgccttc gacatcctgg ccacatcctt catgtacggc agccggacct
      361 tcatcaagta ccccaagggc atccccgatt tcttcaagca gagcttcccc gagggcttca
      421 cctgggagag agtgaccaga tacgaggacg gcggcgtgat caccgtgatg caggacacca
      481 gcctggaaga tggctgcctg gtgtaccatg cccaggtcag gggcgtgaat tttcccagca
      541 acggcgccgt gatgcagaag aaaaccaagg gctgggagcc caacaccgag atgatgtacc
      601 ccgctgacgg cggactgaga ggctacaccc acatggccct gaaggtggac ggcggagggc
      661 acctgagctg cagcttcgtg accacctacc gatccaagaa aaccgtgggc aacatcaaga
      721 tgcccggcat ccacgccgtg gaccaccggc tggaaaggct ggaagagtcc gacaacgaga
      781 tgttcgtggt gcagcgggag cacgccgtgg ccaagttcgc cggcctgggc ggagggtccg
      841 gaaaggacga gctgtaagaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
      901 tctagataac tgatcataat cagccatacc acatttgtag agg
//
