LOCUS       sequence-86767-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..100
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     51..71
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             146..826
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga aaccgtcaga
      121 tccgctagcg ctaccggtcg ccaccatgaa cagcctgatc aaagaaaaca tgcggatgaa
      181 ggtggtgctg gaaggcagcg tgaacggcca ccagttcaag tgcaccggcg agggcgaggg
      241 caacccctac atgggcaccc agaccatgcg gatcaaagtg atcgagggcg gacctctgcc
      301 cttcgccttc gacatcctgg ccacatcctt catgtacggc agccggacct tcatcaagta
      361 ccccaagggc atccccgatt tcttcaagca gagcttcccc gagggcttca cctgggagag
      421 agtgaccaga tacgaggacg gcggcgtgat caccgtgatg caggacacca gcctggaaga
      481 tggctgcctg gtgtaccatg cccaggtcag gggcgtgaat tttcccagca acggcgccgt
      541 gatgcagaag aaaaccaagg gctgggagcc caacaccgag atgatgtacc ccgctgacgg
      601 cggactgaga ggctacaccc acatggccct gaaggtggac ggcggagggc acctgagctg
      661 cagcttcgtg accacctacc gatccaagaa aaccgtgggc aacatcaaga tgcccggcat
      721 ccacgccgtg gaccaccggc tggaaaggct ggaagagtcc gacaacgaga tgttcgtggt
      781 gcagcgggag cacgccgtgg ccaagttcgc cggcctgggc ggagggtccg gactcagatc
      841 taagctgaac cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc
      901 ctgagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
      961 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     1021 tcccccctga acctgaaaca taaaatgaat gcaattgttg ttgtt
//
