LOCUS       sequence-8678-ex        9759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Ricks DM, Kutner R, Zhang XY, Welsh DA, Reiser J
  TITLE     Optimized lentiviral transduction of mouse bone marrow-derived 
            mesenchymal stem cells.
  JOURNAL   Stem Cells Dev. 2008 Jun . 17(3):441-50.
  PUBMED    18513160
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Dev. 2008 Jun . 17(3):441-50."
COMMENT     Sequence Label: pNL-EGFP/CMV/WPREdU3
FEATURES             Location/Qualifiers
     source          1..9759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2710..2913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2864..2884
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2952..2961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2958..2960,2961..2963,2964..3674)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2958..2960,2961..2963,2964..3674)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3003..3024)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3264..3283)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3611..3632
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3694..4282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3747..3767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4165..4176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4357..4589
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6231..6248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6402..6990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6482..6501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7161..7952,7953..8021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7784..7803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8022..8126)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8194..8212
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8263..8285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8371..8509
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8424..8443
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 aataaggaga gaagaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagtta actagatcta
     2401 gagtccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     2761 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     2821 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     2881 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
     2941 cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3001 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3061 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3121 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     3181 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3241 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     3301 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     3361 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     3421 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     3481 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3541 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     3601 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     3661 acgagctgta caagtccgga ctcagatctc gagaatcaac ctctggatta caaaatttgt
     3721 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3781 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3841 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3901 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3961 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4021 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4081 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4141 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4201 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4261 tccctttggg ccgcctcccc gcctgggtac ctttaagacc aatgacttac aaggcagctg
     4321 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaaa
     4381 gaagacaaag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     4441 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4501 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4561 cttttagtca gtgtggaaaa tctctagcac cccccaggag gtagaggttg cagtgagcca
     4621 agatcgcgcc actgcattcc agcctgggca agaaaacaag actgtctaaa ataataataa
     4681 taagttaagg gtattaaata tatttataca tggaggtcat aaaaatatat atatttgggc
     4741 tgggcgcagt ggctcacacc tgcgcccggc cctttgggag gccgaggcag gtggatcacc
     4801 tgagtttggg agttccagac cagcctgacc aacatggaga aaccccttct ctgtgtattt
     4861 ttagtagatt ttattttatg tgtattttat tcacaggtat ttctggaaaa ctgaaactgt
     4921 ttttcctcta ctctgatacc acaagaatca tcagcacaga ggaagacttc tgtgatcaaa
     4981 tgtggtggga gagggaggtt ttcaccagca catgagcagt cagttctgcc gcagactcgg
     5041 cgggtgtcct tcggttcagt tccaacaccg cctgcctgga gagaggtcag accacagggt
     5101 gagggctcag tccccaagac ataaacaccc aagacataaa cacccaacag gtccaccccg
     5161 cctgctgccc aggcagagcc gattcaccaa gacgggaatt aggatagaga aagagtaagt
     5221 cacacagagc cggctgtgcg ggagaacgga gttctattat gactcaaatc agtctcccca
     5281 agcattcggg gatcagagtt tttaaggata acttagtgtg tagggggcca gtgagttgga
     5341 gatgaaagcg tagggagtcg aaggtgtcct tttgcgccga gtcagttcct gggtgggggc
     5401 cacaagatcg gatgagccag tttatcaatc cgggggtgcc agctgatcca tggagtgcag
     5461 ggtctgcaaa atatctcaag cactgattga tcttaggttt tacaatagtg atgttacccc
     5521 aggaacaatt tggggaaggt cagaatcttg tagcctgtag ctgcatgact cctaaaccat
     5581 aatttctttt ttgttttttt ttttttattt ttgagacagg gtctcactct gtcacctagg
     5641 ctggagtgca gtggtgcaat cacagctcac tgcagcctca acgtcgtaag ctcaagcgat
     5701 cctcccacct cagcctgcct ggtagctgag actacaagcg acgccccagt taatttttgt
     5761 atttttggta gaggcagcgt tttgccgtgt ggccctggct ggtctcgaac tcctgggctc
     5821 aagtgatcca gcctcagcct cccaaagtgc tgggacaacc ggggccagtc actgcacctg
     5881 gccctaaacc ataatttcta atcttttggc taatttgtta gtcctacaaa ggcagtctag
     5941 tccccaggca aaaagggggt ttgtttcggg aaagggctgt tactgtcttt gtttcaaact
     6001 ataaactaag ttcctcctaa acttagttcg gcctacaccc aggaatgaac aaggagagct
     6061 tggaggttag aagcacgatg gaattggtta ggtcagatct ctttcactgt ctgagttata
     6121 attttgcaat ggtggttcaa agactgcccg cttctgacac cagtcgctgc attaatgaat
     6181 cggccaacgc gcggggagag gcggtttgcg tattggcgct cttccgcttc ctcgctcact
     6241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     6601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6841 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     8161 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     8221 cttcaagaac tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     8281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     8341 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     8401 cggagtgtac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     8461 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     8521 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     8581 ggggaggcag agattgcagt aagctgagat cgcagcactg cactccagcc tgggcgacag
     8641 agtaagactc tgtctcaaaa ataaaataaa taaatcaatc agatattcca atcttttcct
     8701 ttatttattt atttattttc tattttggaa acacagtcct tccttattcc agaattacac
     8761 atatattcta tttttcttta tatgctccag ttttttttag accttcacct gaaatgtgtg
     8821 tatacaaaat ctaggccagt ccagcagagc ctaaaggtaa aaaataaaat aataaaaaat
     8881 aaataaaatc tagctcactc cttcacatca aaatggagat acagctgtta gcattaaata
     8941 ccaaataacc catcttgtcc tcaataattt taagcgcctc tctccaccac atctaactcc
     9001 tgtcaaaggc atgtgcccct tccgggcgct ctgctgtgct gccaaccaac tggcatgtgg
     9061 actctgcagg gtccctaact gccaagcccc acagtgtgcc ctgaggctgc cccttccttc
     9121 tagcggctgc ccccactcgg ctttgctttc cctagtttca gttacttgcg ttcagccaag
     9181 gtctgaaact aggtgcgcac agagcggtaa gactgcgaga gaaagagacc agctttacag
     9241 ggggtttatc acagtgcacc ctgacagtcg tcagcctcac agggggttta tcacattgca
     9301 ccctgacagt cgtcagcctc acagggggtt tatcacagtg cacccttaca atcattccat
     9361 ttgattcaca atttttttag tctctactgt gcctaacttg taagttaaat ttgatcagag
     9421 gtgtgttccc agaggggaaa acagtatata cagggttcag tactatcgca tttcaggcct
     9481 ccacctgggt cttggaatgt gtcccccgag gggtgatgac tacctcagtt ggatctccac
     9541 aggtcacagt gacacaagat aaccaagaca cctcccaagg ctaccacaat gggccgccct
     9601 ccacgtgcac atggccggag gaactgccat gtcggaggtg caagcacacc tgcgcatcag
     9661 agtccttggt gtggagggag ggaccagcgc agcttccagc catccacctg atgaacagaa
     9721 cctagggaaa gccccagttc tacttacacc aggaaaggc
//
