LOCUS       sequence-86780-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Mito-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..100
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     51..71
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             129..203
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             237..917
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      121 ccgctagcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc tcggcccggc
      181 ggctcccagt gccgcgcgcc aagatccatt cgttggggga tccaccggtc gccaccatga
      241 acagcctgat caaagaaaac atgcggatga aggtggtgct ggaaggcagc gtgaacggcc
      301 accagttcaa gtgcaccggc gagggcgagg gcaaccccta catgggcacc cagaccatgc
      361 ggatcaaagt gatcgagggc ggacctctgc ccttcgcctt cgacatcctg gccacatcct
      421 tcatgtacgg cagccggacc ttcatcaagt accccaaggg catccccgat ttcttcaagc
      481 agagcttccc cgagggcttc acctgggaga gagtgaccag atacgaggac ggcggcgtga
      541 tcaccgtgat gcaggacacc agcctggaag atggctgcct ggtgtaccat gcccaggtca
      601 ggggcgtgaa ttttcccagc aacggcgccg tgatgcagaa gaaaaccaag ggctgggagc
      661 ccaacaccga gatgatgtac cccgctgacg gcggactgag aggctacacc cacatggccc
      721 tgaaggtgga cggcggaggg cacctgagct gcagcttcgt gaccacctac cgatccaaga
      781 aaaccgtggg caacatcaag atgcccggca tccacgccgt ggaccaccgg ctggaaaggc
      841 tggaagagtc cgacaacgag atgttcgtgg tgcagcggga gcacgccgtg gccaagttcg
      901 ccggcctggg cggagggtaa gcggccgcga ctctagatca taatcagcca taccacattt
      961 gtagaggttt tacttgcttt aaaaaacctc ccac
//
