LOCUS       sequence-86781-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..65
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     16..36
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             110..790
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     CDS             815..835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             839..859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             863..883
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
       61 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tgaacagcct
      121 gatcaaagaa aacatgcgga tgaaggtggt gctggaaggc agcgtgaacg gccaccagtt
      181 caagtgcacc ggcgagggcg agggcaaccc ctacatgggc acccagacca tgcggatcaa
      241 agtgatcgag ggcggacctc tgcccttcgc cttcgacatc ctggccacat ccttcatgta
      301 cggcagccgg accttcatca agtaccccaa gggcatcccc gatttcttca agcagagctt
      361 ccccgagggc ttcacctggg agagagtgac cagatacgag gacggcggcg tgatcaccgt
      421 gatgcaggac accagcctgg aagatggctg cctggtgtac catgcccagg tcaggggcgt
      481 gaattttccc agcaacggcg ccgtgatgca gaagaaaacc aagggctggg agcccaacac
      541 cgagatgatg taccccgctg acggcggact gagaggctac acccacatgg ccctgaaggt
      601 ggacggcgga gggcacctga gctgcagctt cgtgaccacc taccgatcca agaaaaccgt
      661 gggcaacatc aagatgcccg gcatccacgc cgtggaccac cggctggaaa ggctggaaga
      721 gtccgacaac gagatgttcg tggtgcagcg ggagcacgcc gtggccaagt tcgccggcct
      781 gggcggaggg tccggactca gatctcgagc tgatccaaaa aagaagagaa aggtagatcc
      841 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtaggatcca ccggatctag
      901 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      961 cccacacctc cccctgaacc tgaaacataa aatg
//
