LOCUS       sequence-86783-e        2480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2480)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1692..1744
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1758..2438
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 gatccgctag caccatggac gacctcgatg ccttactggc agacctggaa tccaccacct
       61 cgcatatctc taagcgaccg gtgtttctaa cagaggaaac gccttactcc tacccaactg
      121 gaaaccatac gtaccaggag attgctgtgc caccgccggt gcccccgccg ccttccagtg
      181 aggccctaaa tggcactgtg attgacccct tagaccagtg gcaacccagc gtgtccagat
      241 acggccacca gcaacctcag tcccagtctc cgatatacag ctctagtgcc aaaagctcca
      301 gtgcctctgt tcctagagac gggctcagct ccccttctcc ccgtgccagt gaggaggaac
      361 acgtgtacag cttcccaaac aagcagaagt ctgcagaacc atctcccaca atgaccagca
      421 cctccctggg cagcaacctc tcggaactgg acaggcttct cctggaactg aatgctgttc
      481 aacataatcc ccccagtggc ttctcagcag atgaagtcag cagaagccca tcactgccca
      541 atgtgaccgg acctcactat gtcatcccag agagcagcag ctctgcagga gggaaggctg
      601 caccccctac aaaagaaaaa ccaaagcgga atggtgggcg tgggattgaa gatgtgcgtc
      661 ccagtgtgga gagcctgctg gatgagctgg agagctctgt gccaagccca gtccctgcaa
      721 tcactgtgag ccaaggggag gtgagcagcc cccagcgtgt caacgccagt cagcagcaga
      781 cccgtatctc tgcttcttca gctacacgag aactggatga gctgatggcg tccctctctg
      841 actttaagtt catggcacag gggaaagccg gcggcagcag ctccccacca tccaccaccc
      901 caaagcctgg cagtcagctg gacaccatgc tgggaagtct gcagtctgac ctgaacaaac
      961 tgggggtagc gacggtcgcc aagggtgtgt gcggagcctg caagaagccg attgctgggc
     1021 aggtagttac agccatgggg aagacctggc accctgagca cttcgtctgc acccactgcc
     1081 aggaggagat tggatcacgg aacttctttg agcgggatgg gcagccctac tgtgagaagg
     1141 actatcacaa cctcttctct cctcgctgct actactgcaa tgggccaatc cttgataaag
     1201 tggtgacggc tttggacagg acgtggcacc ctgaacactt tttctgtgcc cagtgtggag
     1261 ttttctttgg acctgaagga tttcatgaga aggatggcaa agcctattgc cgcaaggact
     1321 actttgacat gtttgctccc aagtgtggag gctgtgcccg ggctatcctg gaaaattaca
     1381 tttctgcctt aaacaccctg tggcaccctg agtgctttgt ctgtcgggaa tgtttcacac
     1441 cgttcatcaa cggcagcttc ttcgagcacg acggccagcc ctactgcgag gtgcattacc
     1501 acgagcgccg cggctcgctg tgctctggct gccagaagcc catcacggga cgctgcatca
     1561 ctgccatggg caagaaattt caccccgaac actttgtctg tgccttctgc ctcaagcagc
     1621 tcaacaaagg aaccttcaaa gaacagaacg acaagcccta ctgtcagaac tgctttctca
     1681 aactcttctg tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
     1741 atccaccggt cgccaccatg aacagcctga tcaaagaaaa catgcggatg aaggtggtgc
     1801 tggaaggcag cgtgaacggc caccagttca agtgcaccgg cgagggcgag ggcaacccct
     1861 acatgggcac ccagaccatg cggatcaaag tgatcgaggg cggacctctg cccttcgcct
     1921 tcgacatcct ggccacatcc ttcatgtacg gcagccggac cttcatcaag taccccaagg
     1981 gcatccccga tttcttcaag cagagcttcc ccgagggctt cacctgggag agagtgacca
     2041 gatacgagga cggcggcgtg atcaccgtga tgcaggacac cagcctggaa gatggctgcc
     2101 tggtgtacca tgcccaggtc aggggcgtga attttcccag caacggcgcc gtgatgcaga
     2161 agaaaaccaa gggctgggag cccaacaccg agatgatgta ccccgctgac ggcggactga
     2221 gaggctacac ccacatggcc ctgaaggtgg acggcggagg gcacctgagc tgcagcttcg
     2281 tgaccaccta ccgatccaag aaaaccgtgg gcaacatcaa gatgcccggc atccacgccg
     2341 tggaccaccg gctggaaagg ctggaagagt ccgacaacga gatgttcgtg gtgcagcggg
     2401 agcacgccgt ggccaagttc gccggcctgg gcggagggta agcggccgcg actctagatc
     2461 ataatcagcc ataccacatt
//
