LOCUS       sequence-86793-e        2485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2485)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1763..2443
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 cagatctcga gctcaagctt cgaattccat ggcggccccc cgcccgtctc ccgcgatccc
       61 cgtttcggtc tcggctccgg ctttttacgc cccgcagaag aagttcggcc ctgtggtggc
      121 cccaaagccc aaagtgaatc ccttccggcc cagggacagc gagcctcccc cggcacccgg
      181 ggcccagcgc gcacagatgg gccgggtggg cgatattccc ccgccgcccc cggaagactt
      241 tcccctgcct ccacctcccc ttgctgggga tggcgacgat gcagagggtg ctctgggagg
      301 tgccttcccg ccgccccctc ccccgatcga ggaatcattt ccccctgcgc ctctggagga
      361 ggagatcttc ccttccccgc cgcctcctcc ggaggaggag ggagggcctg aggcccccat
      421 accgccccca ccacagccca gggagaaggt gagcagtatt gatttggaga tcgactctct
      481 gtcctcactg ctggatgaca tgaccaagaa tgatcctttc aaagcccggg tgtcatctgg
      541 atatgtgccc ccaccagtgg ccactccatt cagttccaag tccagtacca agcctgcagc
      601 cgggggcaca gcacccctgc ctccttggaa gtccccttcc agctcccagc ctctgcccca
      661 ggttccggct ccggctcaga gccagacaca gttccatgtt cagccccagc cccagcccaa
      721 gcctcaggtc caactccatg tccagtccca gacccagcct gtgtctttgg ctaacaccca
      781 gccccgaggg cccccagcct catctccggc tccagcccct aagttttctc cagtgactcc
      841 taagtttact cctgtggctt ccaagttcag tcctggagcc ccaggtggat ctgggtcaca
      901 accaaatcaa aaattggggc accccgaagc tctttctgct ggcacaggct cccctcaacc
      961 tcccagcttc acctatgccc agcagaggga gaagccccga gtgcaggaga agcagcaccc
     1021 cgtgccccca ccggctcaga accaaaacca ggtgcgctcc cctggggccc cagggcccct
     1081 gactctgaag gaggtggagg agctggagca gctgacccag cagctaatgc aggacatgga
     1141 gcatcctcag aggcagaatg tggctgtcaa cgaactctgc ggccgatgcc atcaacccct
     1201 ggcccgggcg cagccagccg tccgcgctct agggcagctg ttccacatcg cctgcttcac
     1261 ctgccaccag tgtgcgcagc agctccaggg ccagcagttc tacagtctgg agggggcgcc
     1321 gtactgcgag ggctgttaca ctgacaccct ggagaagtgt aacacctgcg gggagcccat
     1381 cactgaccgc atgctgaggg ccacgggcaa ggcctatcac ccgcactgct tcacctgtgt
     1441 ggtctgcgcc cgccccctgg agggcacctc cttcatcgtg gaccaggcca accggcccca
     1501 ctgtgtcccc gactaccaca agcagtacgc cccgaggtgc tccgtctgct ctgagcccat
     1561 catgcctgag cctggccgag atgagactgt gcgagtggtc gccctggaca agaacttcca
     1621 catgaagtgt tacaagtgtg aggactgcgg gaagcccctg tcgattgagg cagatgacaa
     1681 tggctgcttc cccctggacg gtcacgtgct ctgtcggaag tgccacactg ctagagccca
     1741 gacggatcca ccggtcgcca ccatgaacag cctgatcaaa gaaaacatgc ggatgaaggt
     1801 ggtgctggaa ggcagcgtga acggccacca gttcaagtgc accggcgagg gcgagggcaa
     1861 cccctacatg ggcacccaga ccatgcggat caaagtgatc gagggcggac ctctgccctt
     1921 cgccttcgac atcctggcca catccttcat gtacggcagc cggaccttca tcaagtaccc
     1981 caagggcatc cccgatttct tcaagcagag cttccccgag ggcttcacct gggagagagt
     2041 gaccagatac gaggacggcg gcgtgatcac cgtgatgcag gacaccagcc tggaagatgg
     2101 ctgcctggtg taccatgccc aggtcagggg cgtgaatttt cccagcaacg gcgccgtgat
     2161 gcagaagaaa accaagggct gggagcccaa caccgagatg atgtaccccg ctgacggcgg
     2221 actgagaggc tacacccaca tggccctgaa ggtggacggc ggagggcacc tgagctgcag
     2281 cttcgtgacc acctaccgat ccaagaaaac cgtgggcaac atcaagatgc ccggcatcca
     2341 cgccgtggac caccggctgg aaaggctgga agagtccgac aacgagatgt tcgtggtgca
     2401 gcgggagcac gccgtggcca agttcgccgg cctgggcgga gggtaagcgg ccgcgactct
     2461 agatcataat cagccatacc acatt
//
