LOCUS       sequence-86794-e        1932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Actin-7
FEATURES             Location/Qualifiers
     source          1..1932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..741
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgtcta agggcgaaga gctgatcaag
       61 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc
      121 acaggtgaag gagaaggcaa tccgtacatg ggaactcaaa ccatgaggat caaagtcatc
      181 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc
      241 cgtactttta tcaagtaccc gaaaggcatt cctgatttct ttaaacagtc ctttcctgag
      301 ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcatgcag
      361 gacaccagcc ttgaggatgg ctgtctcgtt taccacgtcc aagtcagagg ggtaaacttt
      421 ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg
      481 atgtatccag cagatggtgg tctgagggga tacactcata tggcactgaa agttgatggt
      541 ggtggccatc tgtcttgctc tttcgtaaca acttacaggt caaaaaagac cgtcgggaac
      601 atcaagatgc ccggtatcca tgccgttgat caccgcctgg aaaggttaga ggaaagtgac
      661 aatgaaatgt tcgtagtaca acgcgaacac gcagttgcca agttcgccgg gcttggtggt
      721 gggatggacg agctgtacaa gtccggactc agatctcgag ctatggatga tgatatcgcc
      781 gcgctcgtcg tcgacaacgg ctccggcatg tgcaaggccg gcttcgcggg cgacgatgcc
      841 ccccgggccg tcttcccctc catcgtgggg cgccccaggc accagggcgt gatggtgggc
      901 atgggtcaga aggattccta tgtgggcgac gaggcccaga gcaagagagg catcctcacc
      961 ctgaagtacc ccatcgagca cggcatcgtc accaactggg acgacatgga gaaaatctgg
     1021 caccacacct tctacaatga gctgcgtgtg gctcccgagg agcaccccgt gctgctgacc
     1081 gaggcccccc tgaaccccaa ggccaaccgc gagaagatga cccagatcat gtttgagacc
     1141 ttcaacaccc cagccatgta cgttgctatc caggctgtgc tatccctgta cgcctctggc
     1201 cgtaccactg gcatcgtgat ggactccggt gacggggtca cccacactgt gcccatctac
     1261 gaggggtatg ccctccccca tgccatcctg cgtctggacc tggctggccg ggacctgact
     1321 gactacctca tgaagatcct caccgagcgc ggctacagct tcaccaccac ggccgagcgg
     1381 gaaatcgtgc gtgacattaa ggagaagctg tgctacgtcg ccctggactt cgagcaagag
     1441 atggccacgg ctgcttccag ctcctccctg gagaagagct acgagctgcc tgacggccag
     1501 gtcatcacca ttggcaatga gcggttccgc tgccctgagg cactcttcca gccttccttc
     1561 ctgggcatgg agtcctgtgg catccacgaa actaccttca actccatcat gaagtgtgac
     1621 gtggacatcc gcaaagacct gtacgccaac acagtgctgt ctggcggcac caccatgtac
     1681 cctggcattg ccgacaggat gcagaaggag atcactgccc tggcacccag cacaatgaag
     1741 atcaagatca ttgctcctcc tgagcgcaag tactccgtgt ggatcggcgg ctccatcctg
     1801 gcctcgctgt ccaccttcca gcagatgtgg atcagcaagc aggagtatga cgagtccggc
     1861 ccctccatcg tccaccgcaa atgcttctag ggatccaccg gatctagata actgatcata
     1921 atcagccata cc
//
