LOCUS       sequence-86795-e        1970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..747
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgtctaaggg cgaagagctg
       61 atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc
      121 aaatgcacag gtgaaggaga aggcaatccg tacatgggaa ctcaaaccat gaggatcaaa
      181 gtcatcgagg gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat
      241 ggcagccgta cttttatcaa gtacccgaaa ggcattcctg atttctttaa acagtccttt
      301 cctgagggtt ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc
      361 atgcaggaca ccagccttga ggatggctgt ctcgtttacc acgtccaagt cagaggggta
      421 aactttccct ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca
      481 gagatgatgt atccagcaga tggtggtctg aggggataca ctcatatggc actgaaagtt
      541 gatggtggtg gccatctgtc ttgctctttc gtaacaactt acaggtcaaa aaagaccgtc
      601 gggaacatca agatgcccgg tatccatgcc gttgatcacc gcctggaaag gttagaggaa
      661 agtgacaatg aaatgttcgt agtacaacgc gaacacgcag ttgccaagtt cgccgggctt
      721 ggtggtggga tggacgagct gtacaagtcc ggactcagat ctggcagcgg tggaggcagc
      781 gcatccggcg gaagcggaag cgatgatgat atcgccgcgc tcgtcgtcga caacggctcc
      841 ggcatgtgca aggccggctt cgcgggcgac gatgcccccc gggccgtctt cccctccatc
      901 gtggggcgcc ccaggcacca gggcgtgatg gtgggcatgg gtcagaagga ttcctatgtg
      961 ggcgacgagg cccagagcaa gagaggcatc ctcaccctga agtaccccat cgagcacggc
     1021 atcgtcacca actgggacga catggagaaa atctggcacc acaccttcta caatgagctg
     1081 cgtgtggctc ccgaggagca ccccgtgctg ctgaccgagg cccccctgaa ccccaaggcc
     1141 aaccgcgaga agatgaccca gatcatgttt gagaccttca acaccccagc catgtacgtt
     1201 gctatccagg ctgtgctatc cctgtacgcc tctggccgta ccactggcat cgtgatggac
     1261 tccggtgacg gggtcaccca cactgtgccc atctacgagg ggtatgccct cccccatgcc
     1321 atcctgcgtc tggacctggc tggccgggac ctgactgact acctcatgaa gatcctcacc
     1381 gagcgcggct acagcttcac caccacggcc gagcgggaaa tcgtgcgtga cattaaggag
     1441 aagctgtgct acgtcgccct ggacttcgag caagagatgg ccacggctgc ttccagctcc
     1501 tccctggaga agagctacga gctgcctgac ggccaggtca tcaccattgg caatgagcgg
     1561 ttccgctgcc ctgaggcact cttccagcct tccttcctgg gcatggagtc ctgtggcatc
     1621 cacgaaacta ccttcaactc catcatgaag tgtgacgtgg acatccgcaa agacctgtac
     1681 gccaacacag tgctgtctgg cggcaccacc atgtaccctg gcattgccga caggatgcag
     1741 aaggagatca ctgccctggc acccagcaca atgaagatca agatcattgc tcctcctgag
     1801 cgcaagtact ccgtgtggat cggcggctcc atcctggcct cgctgtccac cttccagcag
     1861 atgtggatca gcaagcagga gtatgacgag tccggcccct ccatcgtcca ccgcaaatgc
     1921 ttctagggat ccaccggatc tagataactg atcataatca gccataccac
//
