LOCUS       sequence-86797-e        2613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1856..1865
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1862..2572
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagacc gccatggaag ggaagtggtt
       61 gctgtgtatg ttactggtgc ttggaactgc tattgttgag gctcatgatg gacatgatga
      121 tgatgtgatt gatattgagg atgaccttga cgatgtcatt gaagaggtag aagactcaaa
      181 accagatacc actgctcctc cttcatctcc caaggttact tacaaagctc cagttccaac
      241 aggggaagta tattttgctg attcttttga cagaggaact ctgtcagggt ggattttatc
      301 caaagccaag aaagacgata ccgatgatga aattgccaaa tatgatggaa agtgggaggt
      361 agaggaaatg aaggagtcaa agcttccagg tgataaagga cttgtgttga tgtctcgggc
      421 caagcatcat gccatctctg ctaaactgaa caagcccttc ctgtttgaca ccaagcctct
      481 cattgttcag tatgaggtta atttccaaaa tggaatagaa tgtggtggtg cctatgtgaa
      541 actgctttct aaaacaccag aactcaacct ggatcagttc catgacaaga ccccttatac
      601 gattatgttt ggtccagata aatgtggaga ggactataaa ctgcacttca tcttccgaca
      661 caaaaacccc aaaacgggta tctatgaaga aaaacatgct aagaggccag atgcagatct
      721 gaagacctat tttactgata agaaaacaca tctttacaca ctaatcttga atccagataa
      781 tagttttgaa atactggttg accaatctgt ggtgaatagt ggaaatctgc tcaatgacat
      841 gactcctcct gtaaatcctt cacgtgaaat tgaggaccca gaagaccgga agcccgagga
      901 ttgggatgaa agaccaaaaa tcccagatcc agaagctgtc aagccagatg actgggatga
      961 agatgcccct gctaagattc cagatgaaga ggccacaaaa cccgaaggct ggttagatga
     1021 tgagcctgag tacgtacctg atccagacgc agagaaacct gaggattggg atgaagacat
     1081 ggatggagaa tgggaggctc ctcagattgc caaccctaga tgtgagtcag ctcctggatg
     1141 tggtgtctgg cagcgacctg tgattgacaa ccccaattat aaaggcaaat ggaagcctcc
     1201 tatgattgac aatcccagtt accagggaat ctggaaaccc aggaaaatac caaatccaga
     1261 tttctttgaa gatctggaac ctttcagaat gactcctttt agtgctattg gtttggagct
     1321 gtggtccatg acctctgaca ttttttttga caactttatc atttgtgctg atcgaagaat
     1381 agttgatgat tgggccaatg atggatgggg cctgaagaaa gctgctgatg gggctgctga
     1441 gccaggcgtt gtggggcaga tgaacgaggc agctgaagag cgcccgtggc tgtgggtagt
     1501 ctatattcta actgtagccc ttcctgtgtt cctggttatc ctcttctgct gttctggaaa
     1561 gaaacagacc agtggtatgg agtataagaa aactgatgca cctcaaccgg atgtgaagga
     1621 agaggaagaa gagaaggaag aggaaaagga caagggagat gaggaggagg aaggagaaga
     1681 gaaacttgaa gagaaacaga aaagtgatgc tgaagaagat ggtggcactg tcagtcaaga
     1741 ggaggaagac agaaaaccta aagcagagga ggatgaaatt ttgaacagat caccaagaaa
     1801 cagaaagcca cgaagagagg ggggtgcgtc aggcggcagc ggatctggac cggtcgccac
     1861 catggtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga
     1921 aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca atccgtacat
     1981 gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga
     2041 cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc cgaaaggcat
     2101 tcctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata
     2161 cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc cttgaggatg gctgtctcgt
     2221 ttaccacgtc caagtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa
     2281 gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagggg
     2341 atacactcat atggcactga aagttgatgg tggtggccat ctgtcttgct ctttcgtaac
     2401 aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtatcc atgccgttga
     2461 tcaccgcctg gaaaggttag aggaaagtga caatgaaatg ttcgtagtac aacgcgaaca
     2521 cgcagttgcc aagttcgccg ggcttggtgg tgggatggac gagctgtaca agtaagcggc
     2581 cgcgactcta gatcataatc agccatacca cat
//
