LOCUS       sequence-86798-e        2067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Calreticulin-N-16
FEATURES             Location/Qualifiers
     source          1..2067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1329..2039
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 cgctaccgga ctcagatctc gaggccacca tgctgctatc cgtgccgctg ctgctcggcc
       61 tcctcggcct ggccgtcgcc gagcctgccg tctacttcaa ggagcagttt ctggacggag
      121 acgggtggac ttcccgctgg atcgaatcca aacacaagtc agattttggc aaattcgttc
      181 tcagttccgg caagttctac ggtgacgagg agaaagataa aggtttgcag acaagccagg
      241 atgcacgctt ttatgctctg tcggccagtt tcgagccttt cagcaacaaa ggccagacgc
      301 tggtggtgca gttcacggtg aaacatgagc agaacatcga ctgtgggggc ggctatgtga
      361 agctgtttcc taatagtttg gaccagacag acatgcacgg agactcagaa tacaacatca
      421 tgtttggtcc cgacatctgt ggccctggca ccaagaaggt tcatgtcatc ttcaactaca
      481 agggcaagaa cgtgctgatc aacaaggaca tccgttgcaa ggatgatgag tttacacacc
      541 tgtacacact gattgtgcgg ccagacaaca cctatgaggt gaagattgac aacagccagg
      601 tggagtccgg ctccttggaa gacgattggg acttcctgcc acccaagaag ataaaggatc
      661 ctgatgcttc aaaaccggaa gactgggatg agcgggccaa gatcgatgat cccacagact
      721 ccaagcctga ggactgggac aagcccgagc atatccctga ccctgatgct aagaagcccg
      781 aggactggga tgaagagatg gacggagagt gggaaccccc agtgattcag aaccctgagt
      841 acaagggtga gtggaagccc cggcagatcg acaacccaga ttacaagggc acttggatcc
      901 acccagaaat tgacaacccc gagtattctc ccgatcccag tatctatgcc tatgataact
      961 ttggcgtgct gggcctggac ctctggcagg tcaagtctgg caccatcttt gacaacttcc
     1021 tcatcaccaa cgatgaggca tacgctgagg agtttggcaa cgagacgtgg ggcgtaacaa
     1081 aggcagcaga gaaacaaatg aaggacaaac aggacgagga gcagaggctt aaggaggagg
     1141 aagaagacaa gaaacgcaaa gaggaggagg aggcagagga caaggaggat gatgaggaca
     1201 aagatgagga tgaggaggat gaggaggaca aggaggaaga tgaggaggaa gatgtccccg
     1261 gccaggccaa ggacgagctg gcatccgggg gtgcgtcagg cggcagcgga tctggaccgg
     1321 tcgccaccat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg
     1381 tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc
     1441 cgtacatggg aactcaaacc atgaggatca aagtcatcga gggaggaccc ctgccatttg
     1501 cctttgacat tcttgccacg tcgttcatgt atggcagccg tacttttatc aagtacccga
     1561 aaggcattcc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta
     1621 cgagatacga agatggtgga gtcgtcaccg tcatgcagga caccagcctt gaggatggct
     1681 gtctcgttta ccacgtccaa gtcagagggg taaactttcc ctccaatggt cccgtgatgc
     1741 agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc
     1801 tgaggggata cactcatatg gcactgaaag ttgatggtgg tggccatctg tcttgctctt
     1861 tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtatccatg
     1921 ccgttgatca ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc gtagtacaac
     1981 gcgaacacgc agttgccaag ttcgccgggc ttggtggtgg gatggacgag ctgtacaagt
     2041 aagcggccgc gactctagat cataatc
//
