LOCUS       sequence-86799-e        1335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    528..580
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      588..597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             594..1304
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 cgtcagatcc gctagccacc atgggcccca agcggcggca gctgacgttc cgggagaagt
       61 cgcggatcat ccaggaggtg gaggagaacc cggacctacg caagggcgag atcgcgcggc
      121 gcttcaacat cccgccgtcc acgctgagca cgatcctgaa gaacaagcgc gccatcctgg
      181 cgtcggagcg caagtacggg gtggcctcca cctgccgcaa gaccaacaag ctgtctccct
      241 acgacaagct cgagggcttg ctcatcgcct ggttccagca gatccgcgcc gccggcctgc
      301 cggtcaaggg catcatcctc aaggagaagg cgctgcgcat agccgaggag ctgggcatgg
      361 acgacttcac cgcctccaac ggctggctgg accgcttccg ccggcgccac ggcgtggtgt
      421 cctgcagcgg cgtggcccgc gcccgcgcgc gaaacgctgc cccccgcacc ccggcggcgc
      481 ctgccagtcc ggccgcggtg ccctcggagg gcagtggcgg gagcactctc gagctcaagc
      541 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtgt
      601 ctaagggcga agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg
      661 tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg caatccgtac atgggaactc
      721 aaaccatgag gatcaaagtc atcgagggag gacccctgcc atttgccttt gacattcttg
      781 ccacgtcgtt catgtatggc agccgtactt ttatcaagta cccgaaaggc attcctgatt
      841 tctttaaaca gtcctttcct gagggtttta cttgggaaag agttacgaga tacgaagatg
      901 gtggagtcgt caccgtcatg caggacacca gccttgagga tggctgtctc gtttaccacg
      961 tccaagtcag aggggtaaac tttccctcca atggtcccgt gatgcagaag aagaccaagg
     1021 gttgggagcc taatacagag atgatgtatc cagcagatgg tggtctgagg ggatacactc
     1081 atatggcact gaaagttgat ggtggtggcc atctgtcttg ctctttcgta acaacttaca
     1141 ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtat ccatgccgtt gatcaccgcc
     1201 tggaaaggtt agaggaaagt gacaatgaaa tgttcgtagt acaacgcgaa cacgcagttg
     1261 ccaagttcgc cgggcttggt ggtgggatgg acgagctgta caagtaagcg gccgcgactc
     1321 tagatcataa tcagc
//
