LOCUS       sequence-86801-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      222..231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             228..938
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctggt acccttgcag
       61 cttccagttg tcaggaagag aaggctccca tggatcctgt ggtggtgctg gggctctgtc
      121 tctcctgttt gcttctcctt tcactctgga aacagagcta tgggggaggg aagcttcgaa
      181 ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgtctaagg
      241 gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg
      301 gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca
      361 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt
      421 cgttcatgta tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta
      481 aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag
      541 tcgtcaccgt catgcaggac accagccttg aggatggctg tctcgtttac cacgtccaag
      601 tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg
      661 agcctaatac agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg
      721 cactgaaagt tgatggtggt ggccatctgt cttgctcttt cgtaacaact tacaggtcaa
      781 aaaagaccgt cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa
      841 ggttagagga aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt
      901 tcgccgggct tggtggtggg atggacgagc tgtacaagta agcggccgcg actctagatc
      961 ataatcagcc ataccacatt ttagaaagat ttcc
//
