LOCUS       sequence-86802-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..59
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     10..30
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             104..814
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
       61 gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtgt ctaagggcga
      121 agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg tcaacggcca
      181 ccaattcaaa tgcacaggtg aaggagaagg caatccgtac atgggaactc aaaccatgag
      241 gatcaaagtc atcgagggag gacccctgcc atttgccttt gacattcttg ccacgtcgtt
      301 catgtatggc agccgtactt ttatcaagta cccgaaaggc attcctgatt tctttaaaca
      361 gtcctttcct gagggtttta cttgggaaag agttacgaga tacgaagatg gtggagtcgt
      421 caccgtcatg caggacacca gccttgagga tggctgtctc gtttaccacg tccaagtcag
      481 aggggtaaac tttccctcca atggtcccgt gatgcagaag aagaccaagg gttgggagcc
      541 taatacagag atgatgtatc cagcagatgg tggtctgagg ggatacactc atatggcact
      601 gaaagttgat ggtggtggcc atctgtcttg ctctttcgta acaacttaca ggtcaaaaaa
      661 gaccgtcggg aacatcaaga tgcccggtat ccatgccgtt gatcaccgcc tggaaaggtt
      721 agaggaaagt gacaatgaaa tgttcgtagt acaacgcgaa cacgcagttg ccaagttcgc
      781 cgggcttggt ggtgggatgg acgagctgta caagtccgga ctcagatcta agctgaaccc
      841 tcctgatgag agtggccccg gctgcatgag ctgcaagtgt gtgctctcct gagaattctg
      901 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatca
//
