LOCUS       sequence-86804-e        1195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..741
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             772..1149
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgtcta agggcgaaga gctgatcaag
       61 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc
      121 acaggtgaag gagaaggcaa tccgtacatg ggaactcaaa ccatgaggat caaagtcatc
      181 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc
      241 cgtactttta tcaagtaccc gaaaggcatt cctgatttct ttaaacagtc ctttcctgag
      301 ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcatgcag
      361 gacaccagcc ttgaggatgg ctgtctcgtt taccacgtcc aagtcagagg ggtaaacttt
      421 ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg
      481 atgtatccag cagatggtgg tctgagggga tacactcata tggcactgaa agttgatggt
      541 ggtggccatc tgtcttgctc tttcgtaaca acttacaggt caaaaaagac cgtcgggaac
      601 atcaagatgc ccggtatcca tgccgttgat caccgcctgg aaaggttaga ggaaagtgac
      661 aatgaaatgt tcgtagtaca acgcgaacac gcagttgcca agttcgccgg gcttggtggt
      721 gggatggacg agctgtacaa gtccggactc agatctggag gctccggagg cccagagcca
      781 gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag
      841 aaaggcggca agaagcgcaa gcgcagccgc aaggagagct attccatcta tgtgtacaag
      901 gttctgaagc aggtccaccc tgacaccggc atttcgtcca aggccatggg catcatgaat
      961 tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac
     1021 aacaagcgct cgaccatcac ctccagggag atccagacgg ccgtgcgcct gctgctgcct
     1081 ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc
     1141 gctaagtaag gatccaccgg atctagataa ctgatcataa tcagccatac cacat
//
