LOCUS       sequence-86805-e        3031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-JUP-C-14
FEATURES             Location/Qualifiers
     source          1..3031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..729
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt
       61 atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga
      121 gaaggcaatc cgtacatggg aactcaaacc atgaggatca aagtcatcga gggaggaccc
      181 ctgccatttg cctttgacat tcttgccacg tcgttcatgt atggcagccg tacttttatc
      241 aagtacccga aaggcattcc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg
      301 gaaagagtta cgagatacga agatggtgga gtcgtcaccg tcatgcagga caccagcctt
      361 gaggatggct gtctcgttta ccacgtccaa gtcagagggg taaactttcc ctccaatggt
      421 cccgtgatgc agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca
      481 gatggtggtc tgaggggata cactcatatg gcactgaaag ttgatggtgg tggccatctg
      541 tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc
      601 ggtatccatg ccgttgatca ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc
      661 gtagtacaac gcgaacacgc agttgccaag ttcgccgggc ttggtggtgg gatggacgag
      721 ctgtacaagt ccggaagtgc tgggagcgca agcggaagcg gaggctccgg tgaggtgatg
      781 aacctgatgg agcagcctat caaggtgact gagtggcagc agacatacac ctacgactcg
      841 ggtatccact cgggcgccaa cacctgcgtg ccctccgtca gcagcaaggg catcatggag
      901 gaggatgagg cctgcgggcg ccagtacacg ctcaagaaaa ccaccactta cacccagggg
      961 gtgcccccca gccaaggtga cctggagtac cagatgtcca caacagccag ggccaaacgg
     1021 gtgcgggagg ccatgtgccc tggtgtgtca ggcgaggaca gctcgcttct gctggccacc
     1081 caggtggagg ggcaggccac caacctgcag cgactggccg agccgtccca gctgctcaag
     1141 tcggccattg tgcatctcat caactaccag gacgatgccg agctggccac tcgcgccctg
     1201 cccgagctca ccaaactgct caacgacgag gacccggtgg tggtgaccaa ggcggccatg
     1261 attgtgaacc agctgtcgaa gaaggaggcg tcgcggcggg ccctgatggg ctcgccccag
     1321 ctggtggccg ctgtcgtgcg taccatgcag aataccagcg acctggacac agcccgctgc
     1381 accaccagca tcctgcacaa cctctcccac caccgggagg ggctgctcgc catcttcaag
     1441 tcgggtggca tccctgctct ggtccgcatg ctcagctccc ctgtggagtc ggtcctgttc
     1501 tatgccatca ccacgctgca caacctgctc ctgtaccagg agggcgccaa gatggccgtg
     1561 cgcctggccg acgggctgca aaagatggtg cccctgctca acaagaacaa ccccaagttc
     1621 ctggccatca ccaccgactg cctgcagctc ctggcctacg gcaaccagga gagcaagctg
     1681 atcatcctgg ccaatggtgg gccccaggcc ctcgtgcaga tcatgcgtaa ctacagttat
     1741 gaaaagctgc tctggaccac cagtcgtgtg ctcaaggtgc tatccgtgtg tcccagcaat
     1801 aagcctgcca ttgtggaggc tggtgggatg caggccctgg gcaagcacct gaccagcaac
     1861 agcccccgcc tggtgcagaa ctgcctgtgg accctgcgca acctctcaga tgtggccacc
     1921 aagcaggagg gcctggagag tgtgctgaag attctggtga atcagctgag tgtggatgac
     1981 gtcaacgtcc tcacctgtgc cacgggcaca ctctccaacc tgacatgcaa caacagcaag
     2041 aacaagacgc tggtgacaca gaacagcggt gtggaggctc tcatccatgc catcctgcgt
     2101 gctggtgaca aggacgacat cacggagcct gccgtctgcg ctctgcgcca cctcactagc
     2161 cgccaccctg aggccgagat ggcccagaac tctgtgcgtc tcaactatgg catcccagcc
     2221 atcgtgaagc tgctcaacca gcccaaccag tggccactgg tcaaggcaac catcggcttg
     2281 atcaggaatc tggccctgtg cccagccaac catgccccgc tgcaggaggc agcggtcatc
     2341 ccccgcctcg tccaactgct ggtgaaggcc caccaggatg cccagcgcca cgtagctgca
     2401 ggcacacagc agccctacac ggatggtgtg aggatggagg agattgtgga gggctgcacc
     2461 ggagcactgc acatcctcgc ccgggacccc atgaaccgca tggagatctt ccggctcaac
     2521 accattcccc tgtttgtgca gctcctgtac tcgtcggtgg agaacatcca gcgcgtggct
     2581 gccggggtgc tgtgtgagct ggcccaggac aaggaggcgg ccgacgccat tgatgcagag
     2641 ggggcctcgg ccccactcat ggagttgctg cactcccgca acgagggcac tgccacctac
     2701 gctgctgccg tcctgttccg catctccgag gacaagaacc cagactaccg gaagcgcgtg
     2761 tccgtggagc tcaccaactc cctcttcaag catgacccgg ctgcctggga ggctgcccag
     2821 agcatgattc ccatcaatga gccctatgga gatgacttgg atgccaccta ccgccccatg
     2881 tactccagcg atgtgcccct tgacccgctg gagatgcaca tggacatgga tggagactac
     2941 cccatcgaca cctacagcga cggcctcagg cccccgtacc ccactgcaga ccacatgctg
     3001 gcctagggat ccaccggatc tagataactg a
//
