LOCUS       sequence-86806-e        2109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1363..1372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1369..2079
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gctagtgacg tcagatccgc tagcaccatg agcttcacca ctcgctccac cttctccacc
       61 aactaccggt ccctgggctc tgtccaggcg cccagctacg gcgcccggcc ggtcagcagc
      121 gcggccagcg tctatgcagg cgctgggggc tctggttccc ggatctccgt gtcccgctcc
      181 accagcttca ggggcggcat ggggtccggg ggcctggcca ccgggatagc cgggggtctg
      241 gcaggaatgg gaggcatcca gaacgagaag gagaccatgc aaagcctgaa cgaccgcctg
      301 gcctcttacc tggacagagt gaggagcctg gagaccgaga accggaggct ggagagcaaa
      361 atccgggagc acttggagaa gaagggaccc caggtcagag actggagcca ttacttcaag
      421 atcatcgagg acctgagggc tcagatcttc gcaaatactg tggacaatgc ccgcatcgtt
      481 ctgcagattg acaatgcccg tcttgctgct gatgacttta gagtcaagta tgagacagag
      541 ctggccatgc gccagtctgt ggagaacgac atccatgggc tccgcaaggt cattgatgac
      601 accaatatca cacgactgca gctggagaca gagatcgagg ctctcaagga ggagctgctc
      661 ttcatgaaga agaaccacga agaggaagta aaaggcctac aagcccagat tgccagctct
      721 gggttgaccg tggaggtaga tgcccccaaa tctcaggacc tcgccaagat catggcagac
      781 atccgggccc aatatgacga gctggctcgg aagaaccgag aggagctaga caagtactgg
      841 tctcagcaga ttgaggagag caccacagtg gtcaccacac agtctgctga ggttggagct
      901 gctgagacga cgctcacaga gctgagacgt acagtccagt ccttggagat cgacctggac
      961 tccatgagaa atctgaaggc cagcttggag aacagcctga gggaggtgga ggcccgctac
     1021 gccctacaga tggagcagct caacgggatc ctgctgcacc ttgagtcaga gctggcacag
     1081 acccgggcag agggacagcg ccaggcccag gagtatgagg ccctgctgaa catcaaggtc
     1141 aagctggagg ctgagatcgc cacctaccgc cgcctgctgg aagatggcga ggactttaat
     1201 cttggtgatg ccttggacag cagcaactcc atgcaaacca tccaaaagac caccacccgc
     1261 cggatagtgg atggcaaagt ggtgtctgag accaatgaca ccaaagttct gaggcatcga
     1321 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgtctaag
     1381 ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac
     1441 ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc
     1501 atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg
     1561 tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt
     1621 aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga
     1681 gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa
     1741 gtcagagggg taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg
     1801 gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg
     1861 gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca
     1921 aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa
     1981 aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag
     2041 ttcgccgggc ttggtggtgg gatggacgag ctgtacaagt aagcggccgc gactctagat
     2101 cataatcag
//
