LOCUS       sequence-86810-e        1866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-MAPTau-C-10
FEATURES             Location/Qualifiers
     source          1..1866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..742
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgtct aagggcgaag agctgatcaa
       61 ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg
      121 cacaggtgaa ggagaaggca atccgtacat gggaactcaa accatgagga tcaaagtcat
      181 cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag
      241 ccgtactttt atcaagtacc cgaaaggcat tcctgatttc tttaaacagt cctttcctga
      301 gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcatgca
      361 ggacaccagc cttgaggatg gctgtctcgt ttaccacgtc caagtcagag gggtaaactt
      421 tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat
      481 gatgtatcca gcagatggtg gtctgagggg atacactcat atggcactga aagttgatgg
      541 tggtggccat ctgtcttgct ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa
      601 catcaagatg cccggtatcc atgccgttga tcaccgcctg gaaaggttag aggaaagtga
      661 caatgaaatg ttcgtagtac aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg
      721 tgggatggac gagctgtaca agtccggaag cggcgggtcc gggagcggaa gcgctgagcc
      781 ccgccaggag ttcgaagtga tggaagatca cgctgggacg tacgggttgg gggacaggaa
      841 agatcagggg ggctacacca tgcaccaaga ccaagagggt gacacggacg ctggcctgaa
      901 agctgaagaa gcaggcattg gagacacccc cagcctggaa gacgaagctg ctggtcacgt
      961 gacccaagct cgcatggtca gtaaaagcaa agacgggact ggaagcgatg acaaaaaagc
     1021 caagggggct gatggtaaaa cgaagatcgc cacaccgcgg ggagcagccc ctccaggcca
     1081 gaagggccag gccaacgcca ccaggattcc agcaaaaacc ccgcccgctc caaagacacc
     1141 acccagctct ggtgaacctc caaaatcagg ggatcgcagc ggctacagca gccccggctc
     1201 cccaggcact cccggcagcc gctcccgcac cccgtccctt ccaaccccac ccacccggga
     1261 gcccaagaag gtggcagtgg tccgtactcc acccaagtcg ccgtcttccg ccaagagccg
     1321 cctgcagaca gcccccgtgc ccatgccaga cctgaagaat gtcaagtcca agatcggctc
     1381 cactgagaac ctgaagcacc agccgggagg cgggaaggtg caaatagtct acaaaccagt
     1441 tgacctgagc aaggtgacct ccaagtgtgg ctcattaggc aacatccatc ataaaccagg
     1501 aggtggccag gtggaagtaa aatctgagaa gcttgacttc aaggacagag tccagtcgaa
     1561 gattgggtcc ctggacaata tcacccacgt ccctggcgga ggaaataaaa agattgaaac
     1621 ccacaagctg accttccgcg agaacgccaa agccaagaca gaccacgggg cggagatcgt
     1681 gtacaagtcg ccagtggtgt ctggggacac gtctccacgg catctcagca atgtctcctc
     1741 caccggcagc atcgacatgg tagactcgcc ccagctcgcc acgctagctg acgaggtgtc
     1801 tgcctccctg gccaagcagg gtttgtgagg atccaccgga tctagataac tgatcataat
     1861 cagcca
//
