LOCUS       sequence-86811-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Mito-7
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..96
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             130..840
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 agtgacgtca gatccgctag catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca
       61 ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttggg ggatccaccg
      121 gtcgccacca tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg
      181 gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaat
      241 ccgtacatgg gaactcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt
      301 gcctttgaca ttcttgccac gtcgttcatg tatggcagcc gtacttttat caagtacccg
      361 aaaggcattc ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt
      421 acgagatacg aagatggtgg agtcgtcacc gtcatgcagg acaccagcct tgaggatggc
      481 tgtctcgttt accacgtcca agtcagaggg gtaaactttc cctccaatgg tcccgtgatg
      541 cagaagaaga ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt
      601 ctgaggggat acactcatat ggcactgaaa gttgatggtg gtggccatct gtcttgctct
      661 ttcgtaacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtatccat
      721 gccgttgatc accgcctgga aaggttagag gaaagtgaca atgaaatgtt cgtagtacaa
      781 cgcgaacacg cagttgccaa gttcgccggg cttggtggtg ggatggacga gctgtacaag
      841 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
      901 tttaaaaaac ctcccacacc tcc
//
