LOCUS       sequence-86812-e        6714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..730
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 agcgctaccg gtcgccacca tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg
       61 tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg
      121 agaaggcaat ccgtacatgg gaactcaaac catgaggatc aaagtcatcg agggaggacc
      181 cctgccattt gcctttgaca ttcttgccac gtcgttcatg tatggcagcc gtacttttat
      241 caagtacccg aaaggcattc ctgatttctt taaacagtcc tttcctgagg gttttacttg
      301 ggaaagagtt acgagatacg aagatggtgg agtcgtcacc gtcatgcagg acaccagcct
      361 tgaggatggc tgtctcgttt accacgtcca agtcagaggg gtaaactttc cctccaatgg
      421 tcccgtgatg cagaagaaga ccaagggttg ggagcctaat acagagatga tgtatccagc
      481 agatggtggt ctgaggggat acactcatat ggcactgaaa gttgatggtg gtggccatct
      541 gtcttgctct ttcgtaacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc
      601 cggtatccat gccgttgatc accgcctgga aaggttagag gaaagtgaca atgaaatgtt
      661 cgtagtacaa cgcgaacacg cagttgccaa gttcgccggg cttggtggtg ggatggacga
      721 gctgtacaag tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg
      781 aagcgctcag caggctgcag acaagtacct ctatgtggat aaaaacttca tcaataaccc
      841 gctggcccaa gctgactggg ctgccaagaa gttggtatgg gtgccttcca gcaagaatgg
      901 ctttgaacca gccagcctca aggaggaggt gggagaagag gccattgtag agctggtaga
      961 gaatgggaag aaggtgaagg tgaacaagga cgacatccag aagatgaacc cacccaagtt
     1021 ctccaaggtg gaggacatgg cagagctcac gtgcctcaac gaagcttcgg tgctgcacaa
     1081 cctcaaggag cgatactact cagggcttat ctacacctat tcaggcctgt tctgtgtggt
     1141 catcaaccct tataagaacc tgcccatcta ctcagaggag atcgtggaga tgtacaaggg
     1201 caagaagagg cacgagatgc caccccacat ctacgccatc acagatactg cctaccggag
     1261 catgatgcag gaccgggaag atcagtccat cctgtgcacg ggggagtctg gagcagggaa
     1321 gacagagaac accaagaaag tcatccagta cctggcacat gtggcctcct cacacaagag
     1381 caagaaggac cagggggagt tggagcggca gctgctacag gccaacccta tcctagaggc
     1441 ctttggaaac gccaagacgg tgaagaatga caactcctct cgattcggta aattcattcg
     1501 tatcaacttt gatgtcaatg gctacattgt tggtgccaac attgagactt atcttctgga
     1561 gaaatctcgt gctatccgcc aagccaaaga ggagcggacc ttccacatct tctactacct
     1621 gctgtctggg gccggagaac acctgaagac tgatctcctg ttggagccat acaacaaata
     1681 ccgcttcctg tccaacgggc acgtcaccat ccctgggcag caggacaagg acatgttcca
     1741 ggagacaatg gaggccatga gaattatggg tatcccagag gatgagcaga tgggcttgct
     1801 gcgggtcatc tctggggtcc ttcagcttgg caacattgcc ttcaagaagg agcggaacac
     1861 tgaccaggcg tccatgccgg acaacacagc tgctcaaaag gtgtcccacc tcctggggat
     1921 caatgtgacc gacttcacca gaggcatcct caccccacgc atcaaggtgg gcagagacta
     1981 tgtgcagaag gcgcagacta aagagcaggc tgactttgcc attgaggcct tggccaaggc
     2041 tacctatgag cggatgttcc gctggctggt gcttcgcatc aacaaagctc tggacaagac
     2101 caagaggcag ggcgcctcat ttatcgggat actggacatc gctggctttg aaatctttga
     2161 tctgaactcc ttcgagcagc tgtgcatcaa ctacaccaac gagaagctgc agcagctgtt
     2221 caaccacacc atgttcatcc tggagcagga ggagtaccag cgagagggca tcgagtggaa
     2281 cttcatcgac ttcggcctgg acctgcagcc ctgcatcgac ctcattgaga agccggcggg
     2341 tcccccaggc atcctggccc tgctagatga ggagtgctgg tttcctaagg ccactgacaa
     2401 gagcttcgtg gagaaggtgg tgcaggagca gggcacccac cccaagttcc agaagcccaa
     2461 gcaactgaag gacaaggctg atttctgcat tatccactat gccggcaagg tggactataa
     2521 agctgacgag tggctgatga agaacatgga ccccttgaac gacaacatcg ccacgctgct
     2581 tcaccagtcc tcagacaagt ttgtctctga gctgtggaag gatgtggatc ggatcattgg
     2641 cttggaccaa gtggctggaa tgtccgagac agcactacct ggtgccttca agacccggaa
     2701 gggcatgttc cgtactgtcg gacagctgta caaggagcag ctggccaagc tcatggccac
     2761 gttgaggaat accaacccca acttcgtgcg ctgcatcatt cccaaccatg agaagaaggc
     2821 cggcaaactg gacccgcact tggtgctgga ccagctgcgc tgcaatggcg tccttgaggg
     2881 catccggatc tgccgccagg gctttcccaa cagggtggtc ttccaggagt tccggcagag
     2941 gtatgagatc ctcaccccca actccatccc gaagggcttc atggatggca agcaagcgtg
     3001 tgtgctcatg atcaaagcct tggagcttga cagcaacctg taccgcatcg gccagagcaa
     3061 agtgttcttc cgggcaggag tgctcgccca cctggaggaa gagcgggacc tgaagatcac
     3121 cgatgtcatc attggcttcc aggcctgctg caggggctac ctggccagga aggcctttgc
     3181 caagaggcag caacagctga ccgccatgaa ggtcctacag aggaactgtg ctgcgtacct
     3241 caggctgcgc aactggcagt ggtggaggct cttcaccaag gtcaagcccc tgttgaactc
     3301 aataagacat gaggatgagc tgttagccaa ggaggcggaa ctgacaaagg ttcgagagaa
     3361 acatctggct gcagagaaca ggctgacaga gatggagacg atgcagtctc agctcatggc
     3421 agagaagctg cagcttcagg agcagctgca ggcggagaca gagctgtgtg ccgaggctga
     3481 ggagctccgg gcccgtctga cagcgaagaa gcaggagctg gaggagatat gccatgacct
     3541 ggaggccagg gtggaggagg aggaggagcg ctgccagtac ctgcaggccg agaagaagaa
     3601 gatgcagcag aacatccagg aacttgagga gcagttggag gaggaggaga gcgcccggca
     3661 gaagctgcag cttgagaagg tgaccaccga ggccaagctg aagaaactgg aggaggacca
     3721 gatcatcatg gaggaccaga actgcaaact ggccaaggag aagaaactgc tggaagacag
     3781 agtagctgag ttcactacca acctcatgga agaggaggag aagtccaaga gcctggccaa
     3841 gctcaagaac aagcacgagg caatgatcac cgacctggaa gagcgcctcc gtagggagga
     3901 gaagcagagg caggagttgg agaagacccg tcgcaagctg gagggagact ccacagacct
     3961 cagtgaccag attgctgagc tccaggcgca gatagcagag ctcaagatgc agctggccaa
     4021 gaaggaggag gagttgcagg ctgccttggc cagagtggaa gaagaagctg ctcagaagaa
     4081 tatggccctg aagaagatcc gagaactgga aactcagata tctgagctcc aggaggacct
     4141 ggagtcggag cgagcctcca ggaataaagc cgagaagcag aaacgggatc tgggagagga
     4201 gctggaggcg ctgaagacag agctggagga cacgctggac tccacggctg cccagcagga
     4261 gctgaggtcg aagcgtgagc aggaggtgag catcctgaag aagactctgg aggacgaggc
     4321 caagacccat gaggcccaga tccaggagat gaggcagaag cactcacagg ctgtggagga
     4381 gctggcagat cagttggagc agacgaagcg ggtaaaagct acccttgaga aggcgaagca
     4441 gaccctggag aatgagcggg gagagctggc caatgaggtg aaggccctgc tgcaaggcaa
     4501 gggcgactca gagcacaagc gcaagaaggt ggaggcgcag ctgcaagaac tgcaggtcaa
     4561 gttcagcgag ggagagcgcg tgcgaaccga actggccgac aaggtcacca agctgcaggt
     4621 tgaactggac agtgtgaccg gtctccttag ccagtctgac agcaagtcca gcaagcttac
     4681 gaaggacttc tctgcgctgg agtcccagct tcaggacaca caggagttgc tccaggagga
     4741 gaaccggcag aagctgagcc tgagcaccaa gctcaagcag atggaggatg agaaaaactc
     4801 cttcagggag cagctggagg aggaggagga ggccaagcgc aacttggaga agcagatcgc
     4861 cacgctccat gcccaggtga ccgacatgaa gaagaagatg gaggacggtg tagggtgcct
     4921 ggagactgca gaggaggcga agcggaggct tcagaaggac ttggaaggcc tgagccagcg
     4981 gcttgaggag aaggtggctg cctacgataa gctggagaag accaagacac ggctgcagca
     5041 ggagctggac gacctgctgg ttgacctgga ccaccagcgg cagagcgtct ccaacctgga
     5101 aaagaagcag aagaagttcg accagctcct agccgaggag aagaccatct cggccaagta
     5161 tgcagaggag cgtgaccgag ctgaggctga ggcccgtgag aaggagacaa aggcgctatc
     5221 actggcccgg gcgcttgagg aggccatgga gcagaaggca gagctggagc ggctcaacaa
     5281 gcagttccgc acggagatgg aggacctcat gagctccaag gatgacgtgg gcaagagtgt
     5341 ccacgagctg gagaagtcca agcgggcctt ggagcagcag gtggaggaga tgaagaccca
     5401 gctggaggag ctggaggatg agctgcaggc cacggaggat gccaagctcc gcctggaggt
     5461 gaacctgcag gccatgaagg cccagtttga gcgggatctg cagggccggg atgaacagag
     5521 cgaggagaag aagaagcagc tggtcagaca ggtgcgggag atggaggcgg agctggagga
     5581 tgagaggaag cagcgctcca tggccatggc cgcacgcaag aaactggaga tggatctgaa
     5641 ggacctggag gcacacattg acacagccaa taagaaccgg gaagaggcca tcaaacagct
     5701 gcggaagctt caggcccaga tgaaggactg catgcgggag ctggacgaca cgcgcgcctc
     5761 ccgggaggag atcctggcgc aggccaagga gaatgagaag aagctgaaga gcatggaggc
     5821 cgagatgatt cagctgcagg aggaactggc agctgctgag cgtgctaagc gtcaggccca
     5881 acaggaacgg gacgagctgg ctgatgagat cgccaacagc agtggcaaag gggccctagc
     5941 attagaggag aagcggcgac tggaggcccg cattgccctg ctggaggagg agctggagga
     6001 ggaacagggc aacacggagc tgatcaacga tcggctgaag aaggccaacc tgcagatcga
     6061 ccaaataaac accgacctga acctggaacg cagccacgca cagaagaatg agaatgcgcg
     6121 acagcagctg gaacgccaga acaaggagct caaggccaag ctgcaggaaa tggagagtgc
     6181 tgtcaagtcc aaatacaagg cctccatcgc ggccttggag gccaaaattg cacagctgga
     6241 ggaacagctg gacaacgaga ccaaggagcg ccaggcagcc tccaagcagg tgcgccggac
     6301 ggagaagaag ctgaaggacg tgctgctgca ggtggaggac gagcggagga acgcggaaca
     6361 gttcaaggac caggctgaca aggcgtccac ccgcctgaag cagcttaaac ggcagctaga
     6421 ggaggctgaa gaggaggccc agcgggccaa tgcctcacgc cggaagctgc agcgtgagct
     6481 ggaagatgcc acagagaccg ctgatgctat gaaccgcgag gtcagctccc tgaagaacaa
     6541 actgaggcgt ggggacctgc catttgtcgt gactcgccga attgttcgga aaggcactgg
     6601 cgactgctca gacgaggagg tcgacggtaa agcagatggg gccgatgcca aggcagctga
     6661 ataggaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agat
//
