LOCUS       sequence-86814-e        2224 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..2224
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1464..1473
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1470..2180
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gcttggtgga cgtcagatcc gctagcgatg ccaccatggc caaaagaaat gccgagaagg
       61 aactgacaga taggaattgg gatcaagaag atgaagctga agaggtggga acattctcca
      121 tggccagtga ggaagtcttg aagaatagag ccataaagaa agcaaagcgc agaaatgttg
      181 gatttgaatc tgacactgga ggagccttta aaggttttaa aggtttggtg gtaccttctg
      241 gaggaggacg cttttctgga tttggtagtg gcgctggagg gaagcctctg gaaggactgt
      301 cgaatggaaa caacataacc agtgcccctc ccttcgccag tgcaaaggca gcggcagatc
      361 ccaaggtagc ctttggttct cttgctgcaa atggccctac caccttggtt gataaagttt
      421 caaatcccaa aactaatggg gacagtcagc agccctcctc ctctggcctt gcttccagta
      481 aagcttgtgt cggaaatgcc tatcacaagc agttggccgc cttgaactgc tccgtgcggg
      541 attggatagt gaagcacgtg aatacaaacc ccctctgtga tctgacacct atctttaaag
      601 actatgagaa atatttagca aacattgaac agcaacacgg gaacagtggc aggaattctg
      661 aaagtgaatc taacaaagtg gcagctgaaa cacagtctcc ttcccttttt ggctcaacaa
      721 aattacagca agagtcaacg tttttgtttc atggcaacaa aactgaagat acacctgaca
      781 agaagatgga ggtggcatct gaaaagaaaa cggacccatc atcactagga gcgacaagtg
      841 cctcatttaa tttcggcaag aaagttgata gctctgtttt gggctcatta agctctgtcc
      901 ccctgactgg attttctttc tcccctggaa actccagttt atttggcaaa gatactaccc
      961 agagtaaacc agtctcttca ccatttccca ctaaaccatt ggagggccaa gcagaaggtg
     1021 acagtggtga atgcaaaggt ggagatgaag aagagaatga tgagccaccc aaagtagtag
     1081 ttaccgaagt aaaagaagaa gacgcttttt actccaaaaa gtgtaaactg ttttacaaga
     1141 aagacaatga gtttaaagag aaaggcatag gtactctgca tttaaaacct acagcaaatc
     1201 agaagacaca gcttttggtg cgggcagaca ccaatttagg caacatattg ctgaacgttc
     1261 tgattccacc caatatgcca tgtacgcgaa cagggaagaa taacgttctt atcgtctgtg
     1321 ttccaaatcc accaattgac gagaagaatg ccaccatgcc agtcaccatg ttgattcggg
     1381 taaaaaccag cgaggatgca gacgagttgc acaaaatttt actggagaaa aaggatgcca
     1441 gctccggagg ggatccaccg gtcgccacca tggtgtctaa gggcgaagag ctgatcaagg
     1501 aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca
     1561 caggtgaagg agaaggcaat ccgtacatgg gaactcaaac catgaggatc aaagtcatcg
     1621 agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg tatggcagcc
     1681 gtacttttat caagtacccg aaaggcattc ctgatttctt taaacagtcc tttcctgagg
     1741 gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc gtcatgcagg
     1801 acaccagcct tgaggatggc tgtctcgttt accacgtcca agtcagaggg gtaaactttc
     1861 cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat acagagatga
     1921 tgtatccagc agatggtggt ctgaggggat acactcatat ggcactgaaa gttgatggtg
     1981 gtggccatct gtcttgctct ttcgtaacaa cttacaggtc aaaaaagacc gtcgggaaca
     2041 tcaagatgcc cggtatccat gccgttgatc accgcctgga aaggttagag gaaagtgaca
     2101 atgaaatgtt cgtagtacaa cgcgaacacg cagttgccaa gttcgccggg cttggtggtg
     2161 ggatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     2221 tgta
//
