LOCUS       sequence-86817-e        1436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..740
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgtctaa gggcgaagag ctgatcaagg
       61 aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca
      121 caggtgaagg agaaggcaat ccgtacatgg gaactcaaac catgaggatc aaagtcatcg
      181 agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg tatggcagcc
      241 gtacttttat caagtacccg aaaggcattc ctgatttctt taaacagtcc tttcctgagg
      301 gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc gtcatgcagg
      361 acaccagcct tgaggatggc tgtctcgttt accacgtcca agtcagaggg gtaaactttc
      421 cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat acagagatga
      481 tgtatccagc agatggtggt ctgaggggat acactcatat ggcactgaaa gttgatggtg
      541 gtggccatct gtcttgctct ttcgtaacaa cttacaggtc aaaaaagacc gtcgggaaca
      601 tcaagatgcc cggtatccat gccgttgatc accgcctgga aaggttagag gaaagtgaca
      661 atgaaatgtt cgtagtacaa cgcgaacacg cagttgccaa gttcgccggg cttggtggtg
      721 ggatggacga gctgtacaag tccggactca gatctcgagc catggctagt cgaggcgcaa
      781 caagacccaa cgggccaaat actggaaata aaatatgcca gttcaaacta gtacttctgg
      841 gagagtctgc tgttggcaaa tcaagcctag tgcttcgttt tgtgaagggc caatttcatg
      901 aatttcaaga gagtaccata ggggctgctt ttctaaccca aactgtgtgt cttgatgata
      961 caacagtaaa gtttgaaata tgggatacag ctggtcaaga acgataccat agcttagcac
     1021 caatgtacta cagaggagca caagcagcca tagttgtata tgatatcaca aatgaggagt
     1081 cctttgccag agccaaaaac tgggttaaag aacttcagag gcaagccagt cctaacattg
     1141 taatagcttt atcaggaaac aaggctgatc ttgcaaataa aagagctgtc gatttccagg
     1201 aagcacagtc ctatgcagat gacaacagtt tattattcat ggagacatca gctaaaacat
     1261 cgatgaatgt aaatgaaata ttcatggcaa tagctaaaaa gttgccaaag aacgaaccac
     1321 agaatccagg agcaaattct gccagaggaa gaggagtaga ccttactgaa cccacgcagc
     1381 caaccaggaa tcagtgttgt agtaactaag gatccaccgg atctagataa ctgatc
//
