LOCUS       sequence-86818-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      218..227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             224..934
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcgcc accatgattc acaccaacct
       61 gaagaaaaag ttcagctgct gcgtcctggt ctttcttctg tttgcagtca tctgtgtgtg
      121 gaaggaaaag aagaaaggga gttactatga ttcctttaaa ttgcaaacca aggaattcct
      181 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtgt ctaagggcga
      241 agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg tcaacggcca
      301 ccaattcaaa tgcacaggtg aaggagaagg caatccgtac atgggaactc aaaccatgag
      361 gatcaaagtc atcgagggag gacccctgcc atttgccttt gacattcttg ccacgtcgtt
      421 catgtatggc agccgtactt ttatcaagta cccgaaaggc attcctgatt tctttaaaca
      481 gtcctttcct gagggtttta cttgggaaag agttacgaga tacgaagatg gtggagtcgt
      541 caccgtcatg caggacacca gccttgagga tggctgtctc gtttaccacg tccaagtcag
      601 aggggtaaac tttccctcca atggtcccgt gatgcagaag aagaccaagg gttgggagcc
      661 taatacagag atgatgtatc cagcagatgg tggtctgagg ggatacactc atatggcact
      721 gaaagttgat ggtggtggcc atctgtcttg ctctttcgta acaacttaca ggtcaaaaaa
      781 gaccgtcggg aacatcaaga tgcccggtat ccatgccgtt gatcaccgcc tggaaaggtt
      841 agaggaaagt gacaatgaaa tgttcgtagt acaacgcgaa cacgcagttg ccaagttcgc
      901 cgggcttggt ggtgggatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
      961 tcagccatac cacatt
//
