LOCUS       sequence-86819-e        1237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 559, 
            Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      486..495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             492..1202
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gtgacgtcag atccgctagc gccaccatgg tgggtcggaa cagcgccatc gccgccggtg
       61 tatgcggggc ccttttcatt gggtactgca tctacttcga ccgcaaaaga cgaagtgacc
      121 ccaacttcaa gaacaggctt cgagaacgaa gaaagaaaca gaagcttgcc aaggagagag
      181 ctgggctttc caagttacct gaccttaaag atgctgaagc tgttcagaag ttcttccttg
      241 aagaaataca gcttggtgaa gagttactag ctcaaggtga atatgagaag ggcgtagacc
      301 atctgacaaa tgcaattgct gtgtgtggac agccacagca gttactgcag gtcttacagc
      361 aaactcttcc accaccagtg ttccagatgc ttctgactaa gctcccaaca attagtcaga
      421 gaattgtaag tgctcagagc ttggctgaag atgatgtgga aggcggtagc ggggatccac
      481 cggtcgccac catggtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg
      541 tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca
      601 atccgtacat gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat
      661 ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc
      721 cgaaaggcat tcctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag
      781 ttacgagata cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc cttgaggatg
      841 gctgtctcgt ttaccacgtc caagtcagag gggtaaactt tccctccaat ggtcccgtga
      901 tgcagaagaa gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg
      961 gtctgagggg atacactcat atggcactga aagttgatgg tggtggccat ctgtcttgct
     1021 ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtatcc
     1081 atgccgttga tcaccgcctg gaaaggttag aggaaagtga caatgaaatg ttcgtagtac
     1141 aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg tgggatggac gagctgtaca
     1201 agtaagcggc cgcgactcta gatcataatc agccata
//
