LOCUS       sequence-86820-e        2136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..738
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgtctaagg gcgaagagct gatcaaggaa
       61 aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca
      121 ggtgaaggag aaggcaatcc gtacatggga actcaaacca tgaggatcaa agtcatcgag
      181 ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt
      241 acttttatca agtacccgaa aggcattcct gatttcttta aacagtcctt tcctgagggt
      301 tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt catgcaggac
      361 accagccttg aggatggctg tctcgtttac cacgtccaag tcagaggggt aaactttccc
      421 tccaatggtc ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg
      481 tatccagcag atggtggtct gaggggatac actcatatgg cactgaaagt tgatggtggt
      541 ggccatctgt cttgctcttt cgtaacaact tacaggtcaa aaaagaccgt cgggaacatc
      601 aagatgcccg gtatccatgc cgttgatcac cgcctggaaa ggttagagga aagtgacaat
      661 gaaatgttcg tagtacaacg cgaacacgca gttgccaagt tcgccgggct tggtggtggg
      721 atggacgagc tgtacaagtc cggactcaga tctcgagtgc gtgagtgcat ctccatccac
      781 gttggccagg ctggtgtcca gattggcaat gcctgctggg agctctactg cctggaacac
      841 ggcatccagc ccgatggcca gatgccaagt gacaagacca ttgggggagg agatgactcc
      901 ttcaacacct tcttcagtga gacgggcgct ggcaagcacg tgccccgggc tgtgtttgta
      961 gacttggaac ccacagtcat tgatgaagtt cgcactggca cctaccgcca gctcttccac
     1021 cctgagcagc tcatcacagg caaggaagat gctgccaata actatgcccg agggcactac
     1081 accattggca aggagatcat tgaccttgtg ttggaccgaa ttcgcaagct ggctgaccag
     1141 tgcaccggtc ttcagggctt cttggttttc cacagctttg gtgggggaac tggttctggg
     1201 ttcacctccc tgctcatgga acgtctctca gttgattatg gcaagaagtc caagctggag
     1261 ttctccattt acccagcacc ccaggtttcc acagctgtag ttgagcccta caactccatc
     1321 ctcaccaccc acaccaccct ggagcactct gattgtgcct tcatggtaga caatgaggcc
     1381 atctatgaca tctgtcgtag aaacctcgat atcgagcgcc caacctacac taaccttaac
     1441 cgccttatta gccagattgt gtcctccatc actgcttccc tgagatttga tggagccctg
     1501 aatgttgacc tgacagaatt ccagaccaac ctggtgccct acccccgcat ccacttccct
     1561 ctggccacat atgcccctgt catctctgct gagaaagcct accatgaaca gctttctgta
     1621 gcagagatca ccaatgcttg ctttgagcca gccaaccaga tggtgaaatg tgaccctcgc
     1681 catggtaaat acatggcttg ctgcctgttg taccgtggtg acgtggttcc caaagatgtc
     1741 aatgctgcca ttgccaccat caaaaccaag cgcagcatcc agtttgtgga ttggtgcccc
     1801 actggcttca aggttggcat caactaccag cctcccactg tggtgcctgg tggagacctg
     1861 gccaaggtac agagagctgt gtgcatgctg agcaacacca cagccattgc tgaggcctgg
     1921 gctcgcctgg accacaagtt tgacctgatg tatgccaagc gtgcctttgt tcactggtac
     1981 gtgggtgagg ggatggagga aggcgagttt tcagaggccc gtgaagatat ggctgccctt
     2041 gagaaggatt atgaggaggt tggtgtggat tctgttgaag gagagggtga ggaagaagga
     2101 gaggaatact aaggatccac cggatctaga taactg
//
