LOCUS       sequence-86822-e        6010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..736
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     RBS             2437..2445
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gtctaagggc gaagagctga tcaaggaaaa
       61 tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg
      121 tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg aggatcaaag tcatcgaggg
      181 aggacccctg ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac
      241 ttttatcaag tacccgaaag gcattcctga tttctttaaa cagtcctttc ctgagggttt
      301 tacttgggaa agagttacga gatacgaaga tggtggagtc gtcaccgtca tgcaggacac
      361 cagccttgag gatggctgtc tcgtttacca cgtccaagtc agaggggtaa actttccctc
      421 caatggtccc gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta
      481 tccagcagat ggtggtctga ggggatacac tcatatggca ctgaaagttg atggtggtgg
      541 ccatctgtct tgctctttcg taacaactta caggtcaaaa aagaccgtcg ggaacatcaa
      601 gatgcccggt atccatgccg ttgatcaccg cctggaaagg ttagaggaaa gtgacaatga
      661 aatgttcgta gtacaacgcg aacacgcagt tgccaagttc gccgggcttg gtggtgggat
      721 ggacgagctg tacaagtccg gaagtgctgg gagcgcaagc ggaagcggag gctccggtga
      781 ggaaacagct atatgggaac aacatacagt gacgcttcac agggctcctg gatttggatt
      841 tggaattgca atatctggtg gacgagataa tcctcatttt cagagtgggg aaacgtcaat
      901 agtgatttca gatgtgctga aaggaggacc agctgaagga cagctacagg aaaatgaccg
      961 agttgcaatg gttaacggag tttcaatgga taatgttgaa catgcttttg ctgttcagca
     1021 actaaggaaa agtgggaaaa atgcaaaaat tacaattaga aggaagaaga aagttcaaat
     1081 accagtaagt cgtcctgatc ctgaaccagt atctgataat gaagaagata gttatgatga
     1141 ggaaatacat gatccaagaa gtggccggag tggtgtggtt aacagaagga gtgagaagat
     1201 ttggccgagg gatagaagtg caagtagaga gaggagcttg tccccgcggt cagacaggcg
     1261 gtcagtggct tccagccagc ctgctaaacc tactaaagtc acactggtga aatcccggaa
     1321 aaatgaagaa tatggtcttc gattggcaag ccatatattt gttaaggaaa tttcacaaga
     1381 tagtttggca gcaagagatg gcaatattca agaaggtgat gttgtattga agataaatgg
     1441 tactgtgaca gaaaatatgt cattgacaga tgcaaagaca ttgatagaaa ggtctaaagg
     1501 caaattaaaa atggtagttc aaagagatga acgggctacg ctattgaatg tccctgatct
     1561 ttctgacagc atccactctg ctaatgcctc tgagagagac gacatttcag aaattcagtc
     1621 actggcatca gatcattctg gtcgatcaca cgataggcct ccccgccgca gccggtcacg
     1681 atctcctgac cagcggtcag agccttctga tcattccagg cactcgccgc agcagccaag
     1741 caatggcagt ctgcggagta gagatgaaga gagaatttct aaacctgggg ctgtctcaac
     1801 tcctgtaaag catgctgatg atcacacacc taaaacagtg gaagaagtta cagttgaaag
     1861 aaatgagaaa caaacacctt ctcttccaga accaaagcct gtgtatgccc aagttggcaa
     1921 ccagatgtgg atttacctgt cagtccatct gatggtgtcc tacctaattc aactcatgaa
     1981 gatgggattt cttcggccca gcatgaaatt ggtaaaattc agaaaaggag atagtgtggg
     2041 tttgcggctg gctggtggaa atgatgttgg aatatttgta gctggcgttc tagaagatag
     2101 ccctgcagcc aaggaaggct tagaggaagg tgatcaaatt ctcagggtaa acaacgtaga
     2161 ttttacaaat atcataagag aagaagccgt ccttttcctg cttgacctcc ctaaaggaga
     2221 agaagtgacc atattggctc agaagaagaa ggatgtttat cgtcgcattg tagaatcaga
     2281 tgtaggagat tctttctata ttagaaccca ttttgaatat gaaaaggaat ctccctatgg
     2341 acttagtttt aacaaaggag aggtgttccg tgctgtggat accttgtaca atggaaaact
     2401 gggctcttgg cttgctattc gaattggtaa aaatcataag gaggtagaac gaggcatcat
     2461 ccctaataag aacagagctg agcagctcgc cagtgtacag tatacacttc caaaaacagc
     2521 aggcggagac cgtgctgact tctggagatt cagaggtctt cgcagctcca agagaaatct
     2581 tcgaaaaagc agagaggatt tgtccgctca gcctgttcaa acaaagtttc cagcttatga
     2641 aagagtggtt cttcgagaag ctggatttct gaggcctgta accatttttg gaccaatagc
     2701 tgatgttgcc agagaaaagc tggcaagaga agaaccagat atttatcaaa ttgcaaagag
     2761 tgaaccacga gacgctggaa ctgaccaacg tagctctggc tatattcgcc tgcatacaat
     2821 aaagcaaatc atagatcaag acaaacatgc tttattagat gtaacaccaa atgcagttga
     2881 tcgtcttaac tatgcccagt ggtatccaat tgttgtattt cttaaccctg attctaagca
     2941 aggagtaaaa acaatgagaa tgaggttatg tccagaatct cggaaaagtg ccaggaagtt
     3001 atacgagcga tctcataaac ttgctaaaaa taatcaccat ctttttacaa ctacaattaa
     3061 cttaaattca atgaatgatg gttggtatgg tgcgctgaaa gaagcagttc aacaacagca
     3121 aaaccagctg gtatgggttt ccgagggaaa ggcggatggt gctacaagtg atgaccttga
     3181 tttgcatgat gatcgtctgt cctacctgtc agctccaggt agtgaatact caatgtatag
     3241 cacggacagt agacacactt ctgactatga agacacagac acagaaggcg gggcctacac
     3301 tgatcaagaa ctagatgaaa ctcttaatga tgaggttggg actccaccgg agtctgccat
     3361 tacacggtcc tctgagcctg taagagagga ctcctctgga atgcatcatg aaaaccaaac
     3421 atatcctcct tactcaccac aagcgcagcc acaaccaatt catagaatag actcccctgg
     3481 atttaagcca gcctctcaac agaaagcaga agcttcatct ccagtccctt acctttcgcc
     3541 tgaaacaaac ccagcatcat caacctctgc tgttaatcat aatgtaaatt taactaatgt
     3601 cagactggag gagcccaccc cagctccttc cacctcttac tcaccacaag ctgattcttt
     3661 aagaacacca agtactgagg cagctcacat aatgctaaga gatcaagaac catcattgtc
     3721 gtcgcatgta gatccaacaa aggtgtatag aaaggaccca tatcccgagg aaatgatgag
     3781 gcagaaccat gttttgaaac agccagccgt tagtcaccca gggcacaggc cagacaaaga
     3841 gcctaatctg acctatgaac cccaactccc atacgtagag aaacaagcca gcagagacct
     3901 ggagcagccc acatacagat acgagtcctc aagctatacg gaccagtttt ctcgaaacta
     3961 tgaacatcgt ctgcgatacg aagatcgcgt ccccatgtat gaagaacagt ggtcatatta
     4021 tgatgacaaa cagccctacc catctcggcc accttttgat aatcagcact ctcaagacct
     4081 tgactccaga cagcatcccg aagagtcctc agaacgaggg tactttccac gttttgaaga
     4141 gccagcccct ctgtcttacg acagcagacc acgttacgaa caggcaccta gagcatccgc
     4201 cctgcggcac gaagagcagc cagctcctgg gtatgacaca catggtagac tcagaccgga
     4261 agcccagccc cacccttcag cagggcccaa gcctgcagag tccaagcagt attttgagca
     4321 atattcacgc agttacgagc aagtaccacc ccaaggattt acctctagag caggtcattt
     4381 tgagcctctc catggtgctg cagctgtccc tccgctgata ccttcatctc agcataagcc
     4441 agaagctctg ccttcaaaca ccaaaccact gcctccaccc ccaactcaaa ccgaagaaga
     4501 ggaagatcca gcaatgaagc cacagtctgt actcaccaga gttaagatgt ttgaaaacaa
     4561 aaggtctgca tccttagaga ccaagaagga tgtaaatgac actggcagtt ttaagcctcc
     4621 agaagtagca tctaaacctt caggtgctcc catcattggt cccaaaccca cttctcagaa
     4681 tcaattcagt gaacatgaca aaactctgta caggattcca gaacctcaaa aacctcaact
     4741 gaagccacct gaagatattg ttcggtccaa tcattatgac cctgaagaag atgaagaata
     4801 ttatcgaaaa cagctgtcat actttgaccg aagaagtttt gagaataagc ctcctgcaca
     4861 cattgctgcc agccatctct ccgagcctgc aaagccagct cattctcaga atcaatcaaa
     4921 tttttctagt tattcttcaa agggaaagcc tcctgaagct gatggtgtgg atagatcatt
     4981 tggcgagaaa cgctatgaac ccatccaggc cactccccct cctcctccat tgccctcgca
     5041 gtatgcccag ccatctcagc ctgtcaccag cgcgtctctc cacatacatt ctaagggagc
     5101 acatggtgaa ggtaattcag tgtcattgga ttttcagaat tccttagtgt ccaaaccaga
     5161 cccacctcca tctcagaata agccagcaac tttcagacca ccaaaccgag aagatactgc
     5221 tcaggcagct ttctatcccc agaaaagttt tccagataaa gccccagtta atggaactga
     5281 acagactcag aaaacagtca ctccagcata caatcgattc acaccaaaac catatacaag
     5341 ttctgcccga ccatttgaac gcaagtttga aagtcctaaa ttcaatcaca atcttctgcc
     5401 aagtgaaact gcacataaac ctgacttgtc ttcaaaaact cccacttctc caaaaactct
     5461 tgtgaaatcg cacagtttgg cacagcctcc tgagtttgac agtggagttg aaactttctc
     5521 tatccatgca gagaagccta aatatcaaat aaataatatc agcacagtgc ctaaagctat
     5581 tcctgtgagt ccttcagctg tggaagagga tgaagatgaa gatggtcata ctgtggtggc
     5641 cacagcccga ggcatattta acagcaatgg gggcgtgctg agttccatag aaactggtgt
     5701 tagtataatt atccctcaag gagccattcc cgaaggagtt gagcaggaaa tctatttcaa
     5761 ggtctgccgg gacaacagca tccttccacc tttagataaa gagaaaggtg aaacactgct
     5821 gagtcctttg gtgatgtgtg gtccccatgg cctcaagttc ctgaagcctg tggagctgcg
     5881 cttaccacac tgtgatccta aaacctggca aaacaagtgt cttcccggag atccaaatta
     5941 tctcgttgga gcaaactgtg tttctgtcct tattgaccac ttttaaggat ccaccggatc
     6001 tagataactg
//
