LOCUS       sequence-86823-e        2547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1791..1800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1797..2507
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 ctagtgacgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ccatggcggc cccccgcccg tctcccgcga tccccgtttc ggtctcggct ccggcttttt
      121 acgccccgca gaagaagttc ggccctgtgg tggccccaaa gcccaaagtg aatcccttcc
      181 ggcccaggga cagcgagcct cccccggcac ccggggccca gcgcgcacag atgggccggg
      241 tgggcgatat tcccccgccg cccccggaag actttcccct gcctccacct ccccttgctg
      301 gggatggcga cgatgcagag ggtgctctgg gaggtgcctt cccgccgccc cctcccccga
      361 tcgaggaatc atttccccct gcgcctctgg aggaggagat cttcccttcc ccgccgcctc
      421 ctccggagga ggagggaggg cctgaggccc ccataccgcc cccaccacag cccagggaga
      481 aggtgagcag tattgatttg gagatcgact ctctgtcctc actgctggat gacatgacca
      541 agaatgatcc tttcaaagcc cgggtgtcat ctggatatgt gcccccacca gtggccactc
      601 cattcagttc caagtccagt accaagcctg cagccggggg cacagcaccc ctgcctcctt
      661 ggaagtcccc ttccagctcc cagcctctgc cccaggttcc ggctccggct cagagccaga
      721 cacagttcca tgttcagccc cagccccagc ccaagcctca ggtccaactc catgtccagt
      781 cccagaccca gcctgtgtct ttggctaaca cccagccccg agggccccca gcctcatctc
      841 cggctccagc ccctaagttt tctccagtga ctcctaagtt tactcctgtg gcttccaagt
      901 tcagtcctgg agccccaggt ggatctgggt cacaaccaaa tcaaaaattg gggcaccccg
      961 aagctctttc tgctggcaca ggctcccctc aacctcccag cttcacctat gcccagcaga
     1021 gggagaagcc ccgagtgcag gagaagcagc accccgtgcc cccaccggct cagaaccaaa
     1081 accaggtgcg ctcccctggg gccccagggc ccctgactct gaaggaggtg gaggagctgg
     1141 agcagctgac ccagcagcta atgcaggaca tggagcatcc tcagaggcag aatgtggctg
     1201 tcaacgaact ctgcggccga tgccatcaac ccctggcccg ggcgcagcca gccgtccgcg
     1261 ctctagggca gctgttccac atcgcctgct tcacctgcca ccagtgtgcg cagcagctcc
     1321 agggccagca gttctacagt ctggaggggg cgccgtactg cgagggctgt tacactgaca
     1381 ccctggagaa gtgtaacacc tgcggggagc ccatcactga ccgcatgctg agggccacgg
     1441 gcaaggccta tcacccgcac tgcttcacct gtgtggtctg cgcccgcccc ctggagggca
     1501 cctccttcat cgtggaccag gccaaccggc cccactgtgt ccccgactac cacaagcagt
     1561 acgccccgag gtgctccgtc tgctctgagc ccatcatgcc tgagcctggc cgagatgaga
     1621 ctgtgcgagt ggtcgccctg gacaagaact tccacatgaa gtgttacaag tgtgaggact
     1681 gcgggaagcc cctgtcgatt gaggcagatg acaatggctg cttccccctg gacggtcacg
     1741 tgctctgtcg gaagtgccac actgctagag cccagacgga tccaccggtc gccaccatgg
     1801 tgtctaaggg cgaagagctg atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt
     1861 cggtcaacgg ccaccaattc aaatgcacag gtgaaggaga aggcaatccg tacatgggaa
     1921 ctcaaaccat gaggatcaaa gtcatcgagg gaggacccct gccatttgcc tttgacattc
     1981 ttgccacgtc gttcatgtat ggcagccgta cttttatcaa gtacccgaaa ggcattcctg
     2041 atttctttaa acagtccttt cctgagggtt ttacttggga aagagttacg agatacgaag
     2101 atggtggagt cgtcaccgtc atgcaggaca ccagccttga ggatggctgt ctcgtttacc
     2161 acgtccaagt cagaggggta aactttccct ccaatggtcc cgtgatgcag aagaagacca
     2221 agggttggga gcctaataca gagatgatgt atccagcaga tggtggtctg aggggataca
     2281 ctcatatggc actgaaagtt gatggtggtg gccatctgtc ttgctctttc gtaacaactt
     2341 acaggtcaaa aaagaccgtc gggaacatca agatgcccgg tatccatgcc gttgatcacc
     2401 gcctggaaag gttagaggaa agtgacaatg aaatgttcgt agtacaacgc gaacacgcag
     2461 ttgccaagtt cgccgggctt ggtggtggga tggacgagct gtacaagtaa gcggccgcga
     2521 ctctagatca taatcagcca taccaca
//
