LOCUS       sequence-86824-e        1909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1909)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-Actin-7
FEATURES             Location/Qualifiers
     source          1..1909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..709
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             35..709
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(165..183)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     433..452
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     627..650
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggcc tcctccgagg acgtcatcaa
       61 ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat
      121 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      181 caagggcggc cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc
      241 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      301 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      361 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      421 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
      481 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga
      541 cggcggccac tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct
      601 gcccggcgcc tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac
      661 catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcgcct ccggactcag
      721 atctcgagct atggatgatg atatcgccgc gctcgtcgtc gacaacggct ccggcatgtg
      781 caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca tcgtggggcg
      841 ccccaggcac cagggcgtga tggtgggcat gggtcagaag gattcctatg tgggcgacga
      901 ggcccagagc aagagaggca tcctcaccct gaagtacccc atcgagcacg gcatcgtcac
      961 caactgggac gacatggaga aaatctggca ccacaccttc tacaatgagc tgcgtgtggc
     1021 tcccgaggag caccccgtgc tgctgaccga ggcccccctg aaccccaagg ccaaccgcga
     1081 gaagatgacc cagatcatgt ttgagacctt caacacccca gccatgtacg ttgctatcca
     1141 ggctgtgcta tccctgtacg cctctggccg taccactggc atcgtgatgg actccggtga
     1201 cggggtcacc cacactgtgc ccatctacga ggggtatgcc ctcccccatg ccatcctgcg
     1261 tctggacctg gctggccggg acctgactga ctacctcatg aagatcctca ccgagcgcgg
     1321 ctacagcttc accaccacgg ccgagcggga aatcgtgcgt gacattaagg agaagctgtg
     1381 ctacgtcgcc ctggacttcg agcaagagat ggccacggct gcttccagct cctccctgga
     1441 gaagagctac gagctgcctg acggccaggt catcaccatt ggcaatgagc ggttccgctg
     1501 ccctgaggca ctcttccagc cttccttcct gggcatggag tcctgtggca tccacgaaac
     1561 taccttcaac tccatcatga agtgtgacgt ggacatccgc aaagacctgt acgccaacac
     1621 agtgctgtct ggcggcacca ccatgtaccc tggcattgcc gacaggatgc agaaggagat
     1681 cactgccctg gcacccagca caatgaagat caagatcatt gctcctcctg agcgcaagta
     1741 ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc accttccagc agatgtggat
     1801 cagcaagcag gagtatgacg agtccggccc ctccatcgtc caccgcaaat gcttctaggg
     1861 atccaccgga tctagataac tgatcataat cagccatacc acattgaga
//
