LOCUS       sequence-86825-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      237..246
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             243..920
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             243..920
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(373..391)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     641..660
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     835..858
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctggtaccct tgcagcttcc agttgtcagg aagagaaggc tcccatggat cctgtggtgg
      121 tgctggggct ctgtctctcc tgtttgcttc tcctttcact ctggaaacag agctatgggg
      181 gagggaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
      241 ccatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
      301 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      361 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
      421 tcctgtcccc tcagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc
      481 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      541 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
      601 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      661 ccatgggctg ggaggcctcc accgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      721 agatcaagat gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca
      781 cctacatggc caagaagccc gtgcagctgc ccggcgccta caagaccgac atcaagctgg
      841 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc
      901 gccactccac cggcgcctaa agcggccgcg actctagatc ataatcagcc ataccacatt
      961 tgtagaggtt ttacttgctt taaaaaacct ccca
//
