LOCUS       sequence-86826-e        1657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 584, Emission: 
            607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1657)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-EB3-3
FEATURES             Location/Qualifiers
     source          1..1657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             925..1602
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             925..1602
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1055..1073)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1323..1342
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1517..1540
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgactgcca ccatggccgt caatgtgtac tccacatctg tgaccagtga aaatctgagt
      121 cgccatgata tgcttgcatg ggtcaacgac tccctgcacc tcaactatac caagatagaa
      181 cagctttgtt caggggcagc ctactgccag ttcatggaca tgctcttccc cggctgtgtg
      241 cacttgagga aagtgaagtt ccaggccaaa ctagagcatg aatacatcca caacttcaag
      301 gtgctgcaag cagctttcaa gaagatgggt gttgacaaaa tcattcctgt agagaaatta
      361 gtgaaaggaa aattccaaga taattttgag tttattcagt ggtttaagaa attctttgac
      421 gcaaactatg atggaaagga ttacaaccct ctgctggcgc ggcagggcca ggacgtagcg
      481 ccacctccta acccaggtga tcagatcttc aacaaatcca agaaactcat tggcacagca
      541 gttccacaga ggacgtcccc cacaggccca aaaaacatgc agacctctgg ccggctgagc
      601 aatgtggccc ccccctgcat tctccggaag aatcctccat cagcccgaaa tggcggccat
      661 gagactgatg cccaaattct tgaactcaac caacagctgg tggacttgaa gctgacagtg
      721 gatgggctgg agaaggaacg tgacttctac ttcagcaaac ttcgtgacat cgagctcatc
      781 tgccaggagc atgaaagtga aaacagccct gttatctcag gcatcattgg catcctctat
      841 gccacagagg aaggattcgc accccctgag gacgatgaga ttgaagagca tcaacaagaa
      901 gaccaggacg agtacctgga tccgatggcc tcctccgagg acgtcatcaa ggagttcatg
      961 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1021 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     1081 cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac
     1141 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1201 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1261 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1321 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc
     1381 gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac
     1441 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc
     1501 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     1561 cagtacgagc gcgccgaggg ccgccactcc accggcgcct aagaattcga agcttgatgc
     1621 ggccgcgact ctagatcata atcagccata ccacatt
//
