LOCUS       sequence-86827-e        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-ER-4
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..107
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     58..78
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     5'UTR           135..177
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             186..275
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             288..962
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             288..962
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(418..436)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     686..705
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     880..903
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttggcaccaa aatcaaccgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
       61 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      121 gtcagatccg ctagcttgtt ctttttgcag aagctcagaa taaacgctca actttggcag
      181 ataccatgga cagcaaaggt tcgtcgcaga aagggtcccg cctgctcctg ctgctggtgg
      241 tgtcaaatct actcttgtgc cagggtgtgg tctcctgcag tcgacttatg gcctcctccg
      301 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
      361 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      421 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      481 tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      541 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      601 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      661 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      721 cctccaccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc
      781 tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga
      841 agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca
      901 acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg
      961 ccaaagatga gctctaagga tccaccggat ctagatactg atcatatcag catacacttt
     1021 gagaggttta cttgtttaaa actccacact cccctgacct gaactaatga tgcattgtgt
     1081 gtactgttat gactatatgt acaataagat actccaattc
//
