LOCUS       sequence-86828-e        1196 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1196)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-H2B-6
FEATURES             Location/Qualifiers
     source          1..1196
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             474..1151
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             474..1151
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(604..622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     872..891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1066..1089
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggcct
      481 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
      541 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      601 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      661 ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      721 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      781 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      841 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      901 gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga
      961 tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg
     1021 ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct
     1081 cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca
     1141 ccggcgccta aagcggccgc gactctagat cataatcagc cataccacat tgagaa
//
