LOCUS       sequence-86829-e        4017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4017)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             34..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(164..182)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     432..451
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     626..649
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggcct cctccgagga cgtcatcaag
       61 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcca gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa gctgaaggac
      541 ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgcctc cggactcaga
      721 tctcgagctc aagcttcgaa ttcgggaaac tcgagaacaa agcttgaaaa cggccgcatg
      781 ccagtgtttc atacgcgcac gatcgagagc atcctggagc cggtggcaca gcagatctcc
      841 cacctggtga taatgcacga ggagggcgag gtggacggca aagccattcc tgacctcacc
      901 gcgcccgtgg ccgccgtgca ggcggccgtc agcaacctcg tccgggttgg aaaagagact
      961 gttcaaacca ctgaggatca gattttgaag agagatatgc caccagcatt tattaaggtt
     1021 gagaatgctt gcaccaagct tgtccaggca gctcagatgc ttcagtcaga cccttactca
     1081 gtgcctgctc gagattatct aattgatggg tcaaggggca tcctctctgg aacatcagac
     1141 ctgctcctta ccttcgatga ggctgaggtc cgtaaaatta ttagagtttg caaaggaatt
     1201 ttggaatatc ttacagtggc agaggtggtg gagactatgg aagatttggt cacttacaca
     1261 aagaatcttg ggccaggaat gactaagatg gccaagatga ttgacgagag acagcaggag
     1321 ctcactcacc aggagcaccg agtgatgttg gtgaactcga tgaacaccgt gaaagagttg
     1381 ctgccagttc tcatttcagc tatgaagatt tttgtaacaa ctaaaaactc aaaaaaccaa
     1441 ggcatagagg aagctttaaa aaatcgcaat tttactgtag aaaaaatgag tgctgaaatt
     1501 aatgagataa ttcgtgtgtt acaactcacc tcttgggatg aagatgcctg ggccagcaag
     1561 gacactgaag ccatgaagag agcattggcc tccatagact ccaaactgaa ccaggccaaa
     1621 ggttggctcc gtgaccctag tgcctcccca ggggatgctg gtgagcaggc catcagacag
     1681 atcttagatg aagctggaaa agttggtgaa ctctgtgcag gcaaagaacg cagggagatt
     1741 ctgggaactt gcaaaatgct agggcagatg actgatcaag tggctgacct ccgtgccaga
     1801 ggacaaggat cctcaccggt ggccatgcag aaagctcagc aggtatctca gggtctggat
     1861 gtgctcacag caaaagtgga aaatgcagct cgcaagctgg aagccatgac caactcaaag
     1921 cagagcattg caaagaagat cgatgctgct cagaactggc ttgcagatcc aaatggtgga
     1981 ccggaaggag aagagcagat tcgaggtgct ttggctgaag ctcggaaaat agcagaatta
     2041 tgtgatgatc ctaaagaaag agatgacatt ctacgttccc ttggggaaat atctgctctg
     2101 acttctaaat tagcagatct acgaagacag gggaaaggag attctccaga ggctcgagcc
     2161 ttggccaaac aggtggccac ggccctgcag aacctgcaga ccaaaaccaa ccgggctgtg
     2221 gccaacagca gaccggccaa agcagctgta caccttgagg gcaagattga gcaagcacag
     2281 cggtggattg ataatcccac agtggatgac cgtggagtcg gtcaggctgc catccggggg
     2341 cttgtggccg aagggcatcg tctggctaat gttatgatgg ggccttatcg gcaagatctt
     2401 ctcgccaagt gtgaccgagt ggaccagctg acagcccagc tggctgacct ggctgccaga
     2461 ggggaagggg agagtcctca ggcacgagca cttgcatctc agctccaaga ctccttaaag
     2521 gatctaaaag ctcggatgca ggaggccatg actcaggaag tgtcagatgt tttcagcgat
     2581 accacaactc ccatcaagct gttggcagtg gcagccacgg cgcctcctga tgcgcctaac
     2641 agggaagagg tatttgatga gagggcagct aactttgaaa accattcagg aaagcttggt
     2701 gctacggccg agaaggcggc tgcggttggt actgctaata aatcaacagt ggaaggcatt
     2761 caggcctcag tgaagacggc ccgagaactc acaccccagg tggtctcggc tgctcgtatc
     2821 ttacttagga accctggaaa tcaagctgct tatgaacatt ttgagaccat gaagaaccag
     2881 tggatcgata atgttgaaaa aatgacaggg ctggtggacg aagccattga taccaaatct
     2941 ctgttggatg cttcagaaga agcaattaaa aaagacctgg acaagtgcaa ggtagctatg
     3001 gccaacattc agcctcagat gctggttgct ggggcaacca gtattgctcg tcgggccaac
     3061 cggatcctgc tggtggctaa gagggaggtg gagaattccg aggatcccaa gttccgtgag
     3121 gctgtgaaag ctgcctctga tgaattgagc aaaaccatct ccccgatggt gatggatgca
     3181 aaagctgtgg ctggaaacat ttccgaccct ggactgcaaa agagcttcct ggactcagga
     3241 tatcggatcc tgggagctgt ggccaaggtc agagaagcct tccaacctca ggagcctgac
     3301 ttcccgccgc ctccaccaga ccttgaacaa ctccgactaa cagatgagct tgctcctccc
     3361 aaaccacctc tgcctgaagg tgaggtccct ccacctaggc ctccaccacc agaggaaaag
     3421 gatgaagagt tccctgagca gaaggccggg gaggtgatta accagccaat gatgatggct
     3481 gccagacagc tccatgatga agctcgcaaa tggtccagca agggcaatga catcattgca
     3541 gcagccaagc gcatggctct gctgatggct gagatgtctc ggctggtaag agggggcagt
     3601 ggtaccaagc gggcactcat tcagtgtgcc aaggacatcg ccaaggcctc agatgaggtg
     3661 actcggttgg ccaaggaggt tgccaagcag tgcacagata aacggattag aaccaacctc
     3721 ttacaggtat gtgagcgaat cccaaccata agcacccagc tcaaaatcct gtccacagtg
     3781 aaggccacca tgctgggccg gaccaacatc agtgatgagg agtctgagca ggccacagag
     3841 atgctggttc acaatgccca gaacctcatg cagtctgtga aggagactgt gcgggaagct
     3901 gaagctgctt caatcaaaat tcgaacagat gctggattta cactgcgctg ggttagaaag
     3961 actccctggt accagtaggc ggccgcgact ctagatcata atcagccata ccacatt
//
