LOCUS       sequence-86830-e        2511 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2511)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2511
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1786..1795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1792..2469
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1792..2469
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1922..1940)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2190..2209
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2384..2407
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccatg
       61 gcggcccccc gcccgtctcc cgcgatcccc gtttcggtct cggctccggc tttttacgcc
      121 ccgcagaaga agttcggccc tgtggtggcc ccaaagccca aagtgaatcc cttccggccc
      181 agggacagcg agcctccccc ggcacccggg gcccagcgcg cacagatggg ccgggtgggc
      241 gatattcccc cgccgccccc ggaagacttt cccctgcctc cacctcccct tgctggggat
      301 ggcgacgatg cagagggtgc tctgggaggt gccttcccgc cgccccctcc cccgatcgag
      361 gaatcatttc cccctgcgcc tctggaggag gagatcttcc cttccccgcc gcctcctccg
      421 gaggaggagg gagggcctga ggcccccata ccgcccccac cacagcccag ggagaaggtg
      481 agcagtattg atttggagat cgactctctg tcctcactgc tggatgacat gaccaagaat
      541 gatcctttca aagcccgggt gtcatctgga tatgtgcccc caccagtggc cactccattc
      601 agttccaagt ccagtaccaa gcctgcagcc gggggcacag cacccctgcc tccttggaag
      661 tccccttcca gctcccagcc tctgccccag gttccggctc cggctcagag ccagacacag
      721 ttccatgttc agccccagcc ccagcccaag cctcaggtcc aactccatgt ccagtcccag
      781 acccagcctg tgtctttggc taacacccag ccccgagggc ccccagcctc atctccggct
      841 ccagccccta agttttctcc agtgactcct aagtttactc ctgtggcttc caagttcagt
      901 cctggagccc caggtggatc tgggtcacaa ccaaatcaaa aattggggca ccccgaagct
      961 ctttctgctg gcacaggctc ccctcaacct cccagcttca cctatgccca gcagagggag
     1021 aagccccgag tgcaggagaa gcagcacccc gtgcccccac cggctcagaa ccaaaaccag
     1081 gtgcgctccc ctggggcccc agggcccctg actctgaagg aggtggagga gctggagcag
     1141 ctgacccagc agctaatgca ggacatggag catcctcaga ggcagaatgt ggctgtcaac
     1201 gaactctgcg gccgatgcca tcaacccctg gcccgggcgc agccagccgt ccgcgctcta
     1261 gggcagctgt tccacatcgc ctgcttcacc tgccaccagt gtgcgcagca gctccagggc
     1321 cagcagttct acagtctgga gggggcgccg tactgcgagg gctgttacac tgacaccctg
     1381 gagaagtgta acacctgcgg ggagcccatc actgaccgca tgctgagggc cacgggcaag
     1441 gcctatcacc cgcactgctt cacctgtgtg gtctgcgccc gccccctgga gggcacctcc
     1501 ttcatcgtgg accaggccaa ccggccccac tgtgtccccg actaccacaa gcagtacgcc
     1561 ccgaggtgct ccgtctgctc tgagcccatc atgcctgagc ctggccgaga tgagactgtg
     1621 cgagtggtcg ccctggacaa gaacttccac atgaagtgtt acaagtgtga ggactgcggg
     1681 aagcccctgt cgattgaggc agatgacaat ggctgcttcc ccctggacgg tcacgtgctc
     1741 tgtcggaagt gccacactgc tagagcccag acggatccac cggtcgccac catggcctcc
     1801 tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
     1861 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1921 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtcccct
     1981 cagttccagt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     2041 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     2101 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     2161 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     2221 gaggcctcca ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagatg
     2281 aggctgaagc tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacatggcc
     2341 aagaagcccg tgcagctgcc cggcgcctac aagaccgaca tcaagctgga catcacctcc
     2401 cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc
     2461 ggcgcctaag cggccgcgac tctagatcat aatcagccat accacattga g
//
