LOCUS       sequence-86831-e        1972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 584, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1972)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JRed-Actin-12
FEATURES             Location/Qualifiers
     source          1..1972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(47..52,53..760)
                     /codon_start=1
                     /product="red fluorescent protein KillerRed, a 
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /label=KillerRed
                     /note="mammalian codon-optimized"
                     /translation="GSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNV
                     HAVCETGKLPMSWKPICHLIQYGEPFFARYPNGISHFAQECFPEGLSIDRTVRFENDGT
                     MTSHHTYELDGTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLAF
                     IGFTTADGGLMMGHFDSKMTFNGSRAIKIPGPHFVTIITKQMRDTSDKRDHVCQREVTY
                     AHSVPRITSAIGSDED"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggac gaggatggtt cagagggcgg
       61 ccccgccctg ttccagagcg acatgacctt caaaatcttc atcgacggcg aggtgaacgg
      121 ccagaagttc accatcgtgg ccgacggcag cagcaagttc ccccacggcg acttcaacgt
      181 gcacgccgtg tgcgagaccg gcaagctgcc catgagctgg aagcccatct gccacctgat
      241 ccagtacggc gagcccttct tcgcccgcta ccccaacggc atcagccact tcgcccagga
      301 gtgcttcccc gagggcctga gcatcgaccg caccgtgcgc ttcgagaacg acggcaccat
      361 gaccagccac cacacctacg agctggacgg cacctgcgtg gtcagccgca tcaccgtgaa
      421 ctgcgacggc ttccagcccg acggccccat catgcgcgac cagctggtgg acatcctgcc
      481 caacgagacc cacatgttcc cccacggccc caacgccgtg cgccagctgg ccttcatcgg
      541 cttcaccacc gccgacggcg gcctgatgat gggccacttc gacagcaaga tgaccttcaa
      601 cggcagccgc gccatcaaga tccccggccc ccacttcgtg accatcatca ccaagcagat
      661 gagggacacc agcgacaagc gcgaccacgt gtgccagcgc gaggtgacct acgcccacag
      721 cgtgccccgc atcaccagcg ccatcggtag cgacgaggat tccggactca gatctcgagc
      781 tcaagcttcg aattctgatg atgatatcgc cgcgctcgtc gtcgacaacg gctccggcat
      841 gtgcaaggcc ggcttcgcgg gcgacgatgc cccccgggcc gtcttcccct ccatcgtggg
      901 gcgccccagg caccagggcg tgatggtggg catgggtcag aaggattcct atgtgggcga
      961 cgaggcccag agcaagagag gcatcctcac cctgaagtac cccatcgagc acggcatcgt
     1021 caccaactgg gacgacatgg agaaaatctg gcaccacacc ttctacaatg agctgcgtgt
     1081 ggctcccgag gagcaccccg tgctgctgac cgaggccccc ctgaacccca aggccaaccg
     1141 cgagaagatg acccagatca tgtttgagac cttcaacacc ccagccatgt acgttgctat
     1201 ccaggctgtg ctatccctgt acgcctctgg ccgtaccact ggcatcgtga tggactccgg
     1261 tgacggggtc acccacactg tgcccatcta cgaggggtat gccctccccc atgccatcct
     1321 gcgtctggac ctggctggcc gggacctgac tgactacctc atgaagatcc tcaccgagcg
     1381 cggctacagc ttcaccacca cggccgagcg ggaaatcgtg cgtgacatta aggagaagct
     1441 gtgctacgtc gccctggact tcgagcaaga gatggccacg gctgcttcca gctcctccct
     1501 ggagaagagc tacgagctgc ctgacggcca ggtcatcacc attggcaatg agcggttccg
     1561 ctgccctgag gcactcttcc agccttcctt cctgggcatg gagtcctgtg gcatccacga
     1621 aactaccttc aactccatca tgaagtgtga cgtggacatc cgcaaagacc tgtacgccaa
     1681 cacagtgctg tctggcggca ccaccatgta ccctggcatt gccgacagga tgcagaagga
     1741 gatcactgcc ctggcaccca gcacaatgaa gatcaagatc attgctcctc ctgagcgcaa
     1801 gtactccgtg tggatcggcg gctccatcct ggcctcgctg tccaccttcc agcagatgtg
     1861 gatcagcaag caggagtatg acgagtccgg cccctccatc gtccaccgca aatgcttcta
     1921 gggatccacc ggatctagat aactgatcat aatcagccat accacattga ga
//
