LOCUS       sequence-86833-e        1240 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 584, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1240)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JRed-H2B-6
FEATURES             Location/Qualifiers
     source          1..1240
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..453
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      466..475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(484..489,490..1200)
                     /codon_start=1
                     /product="red fluorescent protein KillerRed, a 
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /label=KillerRed
                     /note="mammalian codon-optimized"
                     /translation="GSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNV
                     HAVCETGKLPMSWKPICHLIQYGEPFFARYPNGISHFAQECFPEGLSIDRTVRFENDGT
                     MTSHHTYELDGTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLAF
                     IGFTTADGGLMMGHFDSKMTFNGSRAIKIPGPHFVTIITKQMRDTSDKRDHVCQREVTY
                     AHSVPRITSAIGSDED"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc
       61 gacggtaccg ccaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc
      121 aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag
      181 gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt
      241 tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca
      301 ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc
      361 cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt
      421 actaaggcca tcaccaagta caccagcgct aaggatccac cggtcgccac catggacgag
      481 gatggttcag agggcggccc cgccctgttc cagagcgaca tgaccttcaa aatcttcatc
      541 gacggcgagg tgaacggcca gaagttcacc atcgtggccg acggcagcag caagttcccc
      601 cacggcgact tcaacgtgca cgccgtgtgc gagaccggca agctgcccat gagctggaag
      661 cccatctgcc acctgatcca gtacggcgag cccttcttcg cccgctaccc caacggcatc
      721 agccacttcg cccaggagtg cttccccgag ggcctgagca tcgaccgcac cgtgcgcttc
      781 gagaacgacg gcaccatgac cagccaccac acctacgagc tggacggcac ctgcgtggtc
      841 agccgcatca ccgtgaactg cgacggcttc cagcccgacg gccccatcat gcgcgaccag
      901 ctggtggaca tcctgcccaa cgagacccac atgttccccc acggccccaa cgccgtgcgc
      961 cagctggcct tcatcggctt caccaccgcc gacggcggcc tgatgatggg ccacttcgac
     1021 agcaagatga ccttcaacgg cagccgcgcc atcaagatcc ccggccccca cttcgtgacc
     1081 atcatcacca agcagatgag ggacaccagc gacaagcgcg accacgtgtg ccagcgcgag
     1141 gtgacctacg cccacagcgt gccccgcatc accagcgcca tcggtagcga cgaggattaa
     1201 agcggccgcg actctagatc ataatcagcc ataccacatt
//
