LOCUS       sequence-86835-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRaspberry-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtggc taccatggtg agcaagggcg aggaggtcat
       61 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt gcatgtacgg
      241 ctccaagggc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaagctgaa
      541 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      601 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctccggact
      721 cagatctcga gtgcgtgagt gcatctccat ccacgttggc caggctggtg tccagattgg
      781 caatgcctgc tgggagctct actgcctgga acacggcatc cagcccgatg gccagatgcc
      841 aagtgacaag accattgggg gaggagatga ctccttcaac accttcttca gtgagacggg
      901 cgctggcaag cacgtgcccc gggctgt
//
