LOCUS       sequence-86837-e        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRaspberry-Mito-7
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..93
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             127..807
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(260..278)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     528..547
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     722..745
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    929..1007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcagat ccgctagcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc
       61 tcggcccggc ggctcccagt gccgcgcgcc aagatccatt cgttggggga tccaccggtg
      121 gctaccatgg tgagcaaggg cgaggaggtc atcaaggagt tcatgcgctt caaggtgcgc
      181 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      241 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      301 tgggacatcc tgtcccctca gtgcatgtac ggctccaagg gctacgtgaa gcaccccgcc
      361 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      421 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      481 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      541 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      601 aagggcgaga tgaagatgag gctgaagctg aaggacggcg gccactacga cgccgaggtc
      661 aagaccacct acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc
      721 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc
      781 gagggccgcc actccaccgg cgcctaagcg gccgcgactc tagatcataa tcagccatac
      841 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      901 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      961 taaagcaata gcatcacaat ttcacaaata agcatttttt tcactgcatt ctagttgtgg
     1021 ttgtccaact catcatgtat ct
//
