LOCUS       sequence-86838-e        1907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1907)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRaspberry-Actin-7
FEATURES             Location/Qualifiers
     source          1..1907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtggc taccatggtg agcaagggcg aggaggtcat
       61 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt gcatgtacgg
      241 ctccaagggc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaagctgaa
      541 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      601 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctccggact
      721 cagatctcga gctatggatg atgatatcgc cgcgctcgtc gtcgacaacg gctccggcat
      781 gtgcaaggcc ggcttcgcgg gcgacgatgc cccccgggcc gtcttcccct ccatcgtggg
      841 gcgccccagg caccagggcg tgatggtggg catgggtcag aaggattcct atgtgggcga
      901 cgaggcccag agcaagagag gcatcctcac cctgaagtac cccatcgagc acggcatcgt
      961 caccaactgg gacgacatgg agaaaatctg gcaccacacc ttctacaatg agctgcgtgt
     1021 ggctcccgag gagcaccccg tgctgctgac cgaggccccc ctgaacccca aggccaaccg
     1081 cgagaagatg acccagatca tgtttgagac cttcaacacc ccagccatgt acgttgctat
     1141 ccaggctgtg ctatccctgt acgcctctgg ccgtaccact ggcatcgtga tggactccgg
     1201 tgacggggtc acccacactg tgcccatcta cgaggggtat gccctccccc atgccatcct
     1261 gcgtctggac ctggctggcc gggacctgac tgactacctc atgaagatcc tcaccgagcg
     1321 cggctacagc ttcaccacca cggccgagcg ggaaatcgtg cgtgacatta aggagaagct
     1381 gtgctacgtc gccctggact tcgagcaaga gatggccacg gctgcttcca gctcctccct
     1441 ggagaagagc tacgagctgc ctgacggcca ggtcatcacc attggcaatg agcggttccg
     1501 ctgccctgag gcactcttcc agccttcctt cctgggcatg gagtcctgtg gcatccacga
     1561 aactaccttc aactccatca tgaagtgtga cgtggacatc cgcaaagacc tgtacgccaa
     1621 cacagtgctg tctggcggca ccaccatgta ccctggcatt gccgacagga tgcagaagga
     1681 gatcactgcc ctggcaccca gcacaatgaa gatcaagatc attgctcctc ctgagcgcaa
     1741 gtactccgtg tggatcggcg gctccatcct ggcctcgctg tccaccttcc agcagatgtg
     1801 gatcagcaag caggagtatg acgagtccgg cccctccatc gtccaccgca aatgcttcta
     1861 gggatccacc ggatctagat aactgatcat aatcagccat accacat
//
