LOCUS       sequence-86840-e        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRaspberry-ER-3
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     167..187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             305..982
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(438..456)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     706..725
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     900..923
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tagtcatcgc tatacatgtg atgcggtttg gcagtacatc aatgggcgtg gatagcggtt
       61 gactcacggg gattccaagt ctccacccca ttgacgtcaa tgggagttgt ttggcaccaa
      121 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      181 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
      241 tagcatgctg ctatccgtgc cgttgctgct cggcctcctc ggcctggccg tcgccgaccg
      301 gtcgatggtg agcaagggcg aggaggtcat caaggagttc atgcgcttca aggtgcgcat
      361 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      421 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
      481 ggacatcctg tcccctcagt gcatgtacgg ctccaagggc tacgtgaagc accccgccga
      541 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      601 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
      661 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
      721 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
      781 gggcgagatg aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa
      841 gaccacctac atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa
      901 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga
      961 gggccgccac tccaccggcg ccaaggacga gctgtaagaa ttctgcagtc gacggtaccg
     1021 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     1081 aggttttact tgctttaaaa acctcccaca cctcccctga acctgaacat aaatgaatgc
     1141 aattgtgtgt taacttgtta ttgcagctta tatggttaca ataagcatag catcacaatt
     1201 tcacaaataa gcatttttca ctgcat
//
