LOCUS       sequence-86841-e        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRaspberry-H2B-6
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             475..1155
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(608..626)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     876..895
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1070..1093
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtggc taccatggtg
      481 agcaagggcg aggaggtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
      541 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      601 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      661 tcccctcagt gcatgtacgg ctccaagggc tacgtgaagc accccgccga catccccgac
      721 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      781 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      841 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      901 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatg
      961 aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
     1021 atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc
     1081 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
     1141 tccaccggcg cctaagcggc cgcgactcta gatcataatc agccatacca cat
//
