LOCUS       sequence-86842-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 574, Emission: 596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mStrawberry-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..43
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             270..980
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(415..433)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     683..702
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggcgtag gcgtgtacgg tggaggtcta tataagcaga gctggtttag tgaaccgtca
       61 gatccgctag cgctaccgga ctcagatctg gtacccttgc agcttccagt tgtcaggaag
      121 agaaggctcc catggatcct gtggtggtgc tggggctctg tctctcctgt ttgcttctcc
      181 tttcactctg gaaacagagc tatgggggag ggaagcttcg aattctgcag tcgacggtac
      241 cgcgggcccg ggatccaccg gtggctacca tggtgagcaa gggcgaggag aataacatgg
      301 ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg
      361 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      421 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctaacc cccaacttca
      481 cctacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      541 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      601 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      661 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      721 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag atgaggctga
      781 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      841 ccgtgcagct gcccggcgcc tacatcgtcg gcatcaagtt ggacatcacc tcccacaacg
      901 aggactacac catcgtggaa ctgtacgaac gcgccgaggg ccgccactcc accggcggca
      961 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacattt
     1021 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc cctga
//
