LOCUS       sequence-86844-e        1224 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 574, Emission: 596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1224)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mStrawberry-H2B-6
FEATURES             Location/Qualifiers
     source          1..1224
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             475..1185
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(620..638)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     888..907
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtggc taccatggtg
      481 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcgc
      541 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      601 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      661 tgggacatcc taacccccaa cttcacctac ggctccaagg cctacgtgaa gcaccccgcc
      721 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      781 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      841 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      901 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      961 aagggcgaga tcaagatgag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     1021 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcggcatc
     1081 aagttggaca tcacctccca caacgaggac tacaccatcg tggaactgta cgaacgcgcc
     1141 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagcggc cgcgactcta
     1201 gatcataatc agccatacca catt
//
