LOCUS       sequence-86855-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Alpha-V-Integrin-25
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    234..286
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      296..305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             302..982
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     703..722
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     897..920
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctccacattg gttaccacta atgtcacctg gggcattcag ccagcgccca tgcctgtgcc
       61 tgtgtgggtg atcattttag cagttctagc aggattgttg ctactggctg ttttggtatt
      121 tgtaatgtac aggatgggct tttttaaacg ggtccggcca cctcaagaag aacaagaaag
      181 ggagcagctt caacctcatg aaaatggtga aggaaactca gaaactccgg gatctcgagc
      241 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccccac cggtcgccac
      301 catggtgagc aagggcgagg aggtcatcaa ggagttcatg cgcttcaagg agcacatgga
      361 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      421 gggcacccag accgccaggc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      481 catcctgtcc cctcagatca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
      541 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      601 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      661 ctacaaggtg aaggtgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      721 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
      781 cgagatgaag atgaggctga ggctgaagga cggcggccac tacgacgccg aggtcaagac
      841 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
      901 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
      961 ccgccactcc accggcgcct aagcggccgc gactctagat cataatcagc cataccacat
     1021 tgaga
//
