LOCUS       sequence-86856-e        1764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1764)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Annexin-12
FEATURES             Location/Qualifiers
     source          1..1764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     435..454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     629..652
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtggct accatggtga gcaagggcga ggaggtcatc
       61 aaggagttca tgcgcttcaa ggagcacatg gagggctccg tgaacggcca cgagttcgag
      121 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccag gctgaaggtg
      181 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagat catgtacggc
      241 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      301 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      361 caggactcct ccctgcagga cggcgagttc atctacaagg tgaaggtgcg cggcaccaac
      421 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      481 cggatgtacc ccgaggacgg cgccctgaag ggcgagatga agatgaggct gaggctgaag
      541 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag
      601 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac
      661 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctccggactc
      721 agatctcgag ctcaagcttc gaattctatg gccatggcaa ccaaaggagg tactgtcaaa
      781 gctgcttcag gattcaatgc catggaagat gcccagaccc tgaggaaggc catgaaaggg
      841 ctcggcaccg atgaagacgc cattattagc gtccttgcct accgcaacac cgcccagcgc
      901 caggagatca ggacagccta caagagcacc atcggcaggg acttgataga cgacctgaag
      961 tcagaactga gtggcaactt cgagcaggtg attgtgggga tgatgacgcc cacggtgctg
     1021 tatgacgtgc aagagctgcg aagggccatg aagggagccg gcactgatga gggctgccta
     1081 attgagatcc tggcctcccg gacccctgag gagatccggc gcataagcca aacctaccag
     1141 cagcaatatg gacggagcct tgaagatgac attcgctctg acacatcgtt catgttccag
     1201 cgagtgctgg tgtctctgtc agctggtggg agggatgaag gaaattatct ggacgatgct
     1261 ctcgtgagac aggatgccca ggacctgtat gaggctggag agaagaaatg ggggacagat
     1321 gaggtgaaat ttctaactgt tctctgttcc cggaaccgaa atcacctgtt gcatgtgttt
     1381 gatgaataca aaaggatatc acagaaggat attgaacaga gtattaaatc tgaaacatct
     1441 ggtagctttg aagatgctct gctggctata gtaaagtgca tgaggaacaa atctgcatat
     1501 tttgctgaaa agctctataa atcgatgaag ggcttgggca ccgatgataa caccctcatc
     1561 agagtgatgg tttctcgagc agaaattgac atgttggata tccgggcaca cttcaagaga
     1621 ctctatggaa agtctctgta ctcgttcatc aagggtgaca catctggaga ctacaggaaa
     1681 gtactgcttg ttctctgtgg aggagatgat taaggatcca ccggatctag ataactgatc
     1741 ataatcagcc ataccacatt gtag
//
