LOCUS       sequence-86857-e        3122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3122)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..696
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     420..439
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     614..637
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      751..760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             802..837
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 gcgctaccgg tggctaccat ggtgagcaag ggcgaggagg tcatcaagga gttcatgcgc
       61 ttcaaggagc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      121 gagggccgcc cctacgaggg cacccagacc gccaggctga aggtgaccaa gggtggcccc
      181 ctgcccttcg cctgggacat cctgtcccct cagatcatgt acggctccaa ggcctacgtg
      241 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      301 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      361 caggacggcg agttcatcta caaggtgaag gtgcgcggca ccaacttccc ctccgacggc
      421 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      481 gacggcgccc tgaagggcga gatgaagatg aggctgaggc tgaaggacgg cggccactac
      541 gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc cggcgcctac
      601 aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
      661 tacgagcgcg ccgagggccg ccactccacc ggcgcctccg gactcagatc tcgagctcaa
      721 gcttcgaatt ctgcagtcga cggtaccgcg gccaccatgg ctactcaagc tgacctgatg
      781 gagttggaca tggccatgga gccggacaga aaagctgctg tcagccactg gcagcagcag
      841 tcttacttgg attctggaat ccattctggt gccaccacca cagctccttc cctgagtggc
      901 aagggcaacc ctgaggaaga agatgttgac acctcccaag tcctttatga atgggagcaa
      961 ggcttttccc agtccttcac gcaagagcaa gtagctgata ttgacgggca gtatgcaatg
     1021 actagggctc agagggtccg agctgccatg ttccctgaga cgctagatga gggcatgcag
     1081 atcccatcca cgcagtttga cgctgctcat cccactaatg tccagcgctt ggctgaacca
     1141 tcacagatgt tgaaacatgc agttgtcaat ttgattaact atcaggatga cgcggaactt
     1201 gccacacgtg caattcctga gctgacaaaa ctgctaaacg atgaggacca ggtggtagtt
     1261 aataaagctg ctgttatggt ccatcagctt tccaaaaagg aagcttccag acatgccatc
     1321 atgcgctccc ctcagatggt gtctgccatt gtacgcacca tgcagaatac aaatgatgta
     1381 gagacagctc gttgtactgc tgggactctg cacaaccttt ctcaccaccg cgagggcttg
     1441 ctggccatct ttaagtctgg tggcatccca gcgctggtga aaatgcttgg gtcaccagtg
     1501 gattctgtac tgttctacgc catcacgaca ctgcataatc tcctgctcca tcaggaagga
     1561 gctaaaatgg cagtgcgcct agctggtgga ctgcagaaaa tggttgcttt gctcaacaaa
     1621 acaaacgtga aattcttggc tattacaaca gactgccttc agatcttagc ttatggcaat
     1681 caagagagca agctcatcat tctggccagt ggtggacccc aagccttagt aaacataatg
     1741 aggacctaca cttatgagaa gcttctgtgg accacaagca gagtgctgaa agtgctgtct
     1801 gtctgctcta gcaacaagcc ggccattgta gaagctggtg ggatgcaggc actggggctt
     1861 catctgacag acccaagtca gcgacttgtt caaaactgtc tttggactct cagaaacctt
     1921 tcagatgcag cgactaagca ggaagggatg gaaggcctcc ttgggactct agtgcagctt
     1981 ctgggttccg atgatataaa tgtggtcacc tgtgcagctg gaattctctc taacctcact
     2041 tgcaataatt acaaaaacaa gatgatggtg tgccaagtgg gtggcataga ggctcttgta
     2101 cgcaccgtcc ttcgtgctgg tgacagggaa gacatcactg agcctgccat ctgtgctctt
     2161 cgtcatctga ccagccggca tcaggaagcc gagatggccc agaatgccgt tcgccttcat
     2221 tatggactgc ctgttgtggt taaactcctg cacccaccat cccactggcc tctgataaag
     2281 gcaactgttg gattgattcg aaaccttgcc ctttgcccag caaatcatgc gcctttgcgg
     2341 gaacagggtg ctattccacg actagttcag ctgcttgtac gagcacatca ggacacccaa
     2401 cggcgcacct ccatgggtgg aacgcagcag cagtttgtgg agggcgtgcg catggaggag
     2461 atagtagaag ggtgtactgg agctctccac atccttgctc gggacgttca caaccggatt
     2521 gtaatccgag gactcaatac cattccattg tttgtgcagt tgctttattc tcccattgaa
     2581 aatatccaaa gagtagctgc aggggtcctc tgtgaacttg ctcaggacaa ggaggctgca
     2641 gaggccattg aagctgaggg agccacagct cccctgacag agttactcca ctccaggaat
     2701 gaaggcgtgg caacatacgc agctgctgtc ctattccgaa tgtctgagga caagccacag
     2761 gattacaaga agcggctttc agtcgagctg accagttccc tcttcaggac agagccaatg
     2821 gcttggaatg agactgcaga tcttggactg gacattggtg cccagggaga agcccttgga
     2881 tatcgccagg atgatcccag ctaccgttct tttcactctg gtggatacgg ccaggatgcc
     2941 ttggggatgg accctatgat ggagcatgag atgggtggcc accaccctgg tgctgactat
     3001 ccagttgatg ggctgcctga tctgggacac gcccaggacc tcatggatgg gctgccccca
     3061 ggtgatagca atcagctggc ctggtttgat actgacctgt aaaagcttgg atccaccgga
     3121 at
//
