LOCUS       sequence-86859-e        2554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2554)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1846..1855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1852..2532
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     2253..2272
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2447..2470
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctagcgctac cggactcaga tctcgagacc gccatggaag ggaagtggtt gctgtgtatg
       61 ttactggtgc ttggaactgc tattgttgag gctcatgatg gacatgatga tgatgtgatt
      121 gatattgagg atgaccttga cgatgtcatt gaagaggtag aagactcaaa accagatacc
      181 actgctcctc cttcatctcc caaggttact tacaaagctc cagttccaac aggggaagta
      241 tattttgctg attcttttga cagaggaact ctgtcagggt ggattttatc caaagccaag
      301 aaagacgata ccgatgatga aattgccaaa tatgatggaa agtgggaggt agaggaaatg
      361 aaggagtcaa agcttccagg tgataaagga cttgtgttga tgtctcgggc caagcatcat
      421 gccatctctg ctaaactgaa caagcccttc ctgtttgaca ccaagcctct cattgttcag
      481 tatgaggtta atttccaaaa tggaatagaa tgtggtggtg cctatgtgaa actgctttct
      541 aaaacaccag aactcaacct ggatcagttc catgacaaga ccccttatac gattatgttt
      601 ggtccagata aatgtggaga ggactataaa ctgcacttca tcttccgaca caaaaacccc
      661 aaaacgggta tctatgaaga aaaacatgct aagaggccag atgcagatct gaagacctat
      721 tttactgata agaaaacaca tctttacaca ctaatcttga atccagataa tagttttgaa
      781 atactggttg accaatctgt ggtgaatagt ggaaatctgc tcaatgacat gactcctcct
      841 gtaaatcctt cacgtgaaat tgaggaccca gaagaccgga agcccgagga ttgggatgaa
      901 agaccaaaaa tcccagatcc agaagctgtc aagccagatg actgggatga agatgcccct
      961 gctaagattc cagatgaaga ggccacaaaa cccgaaggct ggttagatga tgagcctgag
     1021 tacgtacctg atccagacgc agagaaacct gaggattggg atgaagacat ggatggagaa
     1081 tgggaggctc ctcagattgc caaccctaga tgtgagtcag ctcctggatg tggtgtctgg
     1141 cagcgacctg tgattgacaa ccccaattat aaaggcaaat ggaagcctcc tatgattgac
     1201 aatcccagtt accagggaat ctggaaaccc aggaaaatac caaatccaga tttctttgaa
     1261 gatctggaac ctttcagaat gactcctttt agtgctattg gtttggagct gtggtccatg
     1321 acctctgaca ttttttttga caactttatc atttgtgctg atcgaagaat agttgatgat
     1381 tgggccaatg atggatgggg cctgaagaaa gctgctgatg gggctgctga gccaggcgtt
     1441 gtggggcaga tgaacgaggc agctgaagag cgcccgtggc tgtgggtagt ctatattcta
     1501 actgtagccc ttcctgtgtt cctggttatc ctcttctgct gttctggaaa gaaacagacc
     1561 agtggtatgg agtataagaa aactgatgca cctcaaccgg atgtgaagga agaggaagaa
     1621 gagaaggaag aggaaaagga caagggagat gaggaggagg aaggagaaga gaaacttgaa
     1681 gagaaacaga aaagtgatgc tgaagaagat ggtggcactg tcagtcaaga ggaggaagac
     1741 agaaaaccta aagcagagga ggatgaaatt ttgaacagat caccaagaaa cagaaagcca
     1801 cgaagagagg ggggtgcgtc aggcggcagc ggatctggac cggtcgccac catggtgagc
     1861 aagggcgagg aggtcatcaa ggagttcatg cgcttcaagg agcacatgga gggctccgtg
     1921 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1981 accgccaggc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     2041 cctcagatca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     2101 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2161 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     2221 aaggtgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     2281 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatgaag
     2341 atgaggctga ggctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg
     2401 gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc
     2461 tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc
     2521 accggcgcct aagcggccgc gactctagat cata
//
