LOCUS       sequence-86861-e        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..704
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     428..447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     622..645
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atccgctagc gctaccggtg gctaccatgg tgagcaaggg cgaggaggtc atcaaggagt
       61 tcatgcgctt caaggagcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caggctgaag gtgaccaagg
      181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gatcatgtac ggctccaagg
      241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      361 cctccctgca ggacggcgag ttcatctaca aggtgaaggt gcgcggcacc aacttcccct
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      481 accccgagga cggcgccctg aagggcgaga tgaagatgag gctgaggctg aaggacggcg
      541 gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg cagctgcccg
      601 gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgcctccgga ctcagatctg
      721 gcagcagtgg aggttctggg ggcaaatacg tagactcgga gggacatctc tacaccgttc
      781 ccatccggga acagggcaac atctacaagc ccaacaacaa ggccatggca gacgagctga
      841 gcgagaagca agtgtacgac gcgcacacca aggagatcga cctggtcaac cgcgacccta
      901 aacacctcaa cgatgacgtg gtcaagattg actttgaaga tgtgattgca gaaccagaag
      961 ggacacacag ttttgacggc atttggaagg ccagcttcac caccttcact gtgacgaaat
     1021 actggtttta ccgcttgctg tctgccctct ttggcatccc gatggcactc atctggggca
     1081 tttacttcgc cattctctct ttcctgcaca tctgggcagt tgtaccatgc attaagagct
     1141 tcctgattga gattcagtgc atcagccgtg tctattccat ctacgtccac accgtctgtg
     1201 acccactctt tgaagctgtt gggaaaatat tcagcaatgt ccgcatcaac ttgcagaaag
     1261 aaatataaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa
     1321 ctgatcataa tcagccatac cacatt
//
