LOCUS       sequence-86863-e        2398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2398)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..98
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1677..2357
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     2078..2097
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2272..2295
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcatgg
       61 ggagcagcaa gagcaagccc aaggacccca gccagcgccg gcgcagcctg gagccacccg
      121 acagcaccca ccacggggga ttcccagcct cgcagacccc caacaagaca gcagcccccg
      181 acacgcaccg cacccccagc cgctcctttg ggaccgtggc caccgagccc aagctcttcg
      241 ggggcttcaa cacttctgac accgtcacgt cgccgcagcg tgccggggca ctggctggcg
      301 gcgtcaccac tttcgtggct ctctatgact acgagtcccg gactgaaacg gacttgtcct
      361 tcaagaaagg agaacgcctg cagattgtca acaacacgga aggtgactgg tggctggctc
      421 attccctcac tacaggacag acgggctaca tccccagtaa ctatgtcgcg ccctcagact
      481 ccatccaggc tgaagagtgg tactttggga agatcactcg tcgggagtcc gagcggctgc
      541 tgctcaaccc cgaaaacccc cggggaacct tcttggtccg ggagagcgag acgacaaaag
      601 gtgcctattg cctctccgtt tctgactttg acaacgccaa ggggctcaat gtgaagcact
      661 acaagatccg caagctggac agcggcggct tctacatcac ctcacgcaca cagttcagca
      721 gcctgcagca gctggtggcc tactactcca aacatgctga tggcttgtgc caccgcctga
      781 ccaacgtctg ccccacgtcc aagccccaga cccagggact cgccaaggac gcgtgggaaa
      841 tcccccggga gtcgctgcgg ctggaggtga agctggggca gggctgcttt ggagaggtct
      901 ggatggggac ctggaacggc accaccagag tggccataaa gactctgaag cccggcacca
      961 tgtccccgga ggccttcctg caggaagccc aagtgatgaa gaagctccgg catgagaagc
     1021 tggttcagct gtacgcagtg gtgtcggaag agcccatcta catcgtcact gagtacatga
     1081 gcaaggggag cctcctggat ttcctgaagg gagagatggg caagtacctg cggctgccac
     1141 agctcgtcga tatggctgct cagattgcat ccggcatggc ctatgtggag aggatgaact
     1201 acgtgcaccg agacctgcgg gcggccaaca tcctggtggg ggagaacctg gtgtgcaagg
     1261 tggctgactt tgggctggca cgcctcatcg aggacaacga gtacacagca cggcaaggtg
     1321 ccaagttccc catcaagtgg acagcccccg aggcagccct ctatggccgg ttcaccatca
     1381 agtcggatgt ctggtccttc ggcatcctgc tgactgagct gaccaccaag ggccgggtgc
     1441 catacccagg gatggtcaac agggaggtgc tggaccaggt ggagaggggc taccgcatgc
     1501 cctgcccgcc cgagtgcccc gagtcgctgc atgacctcat gtgccagtgc tggcggaagg
     1561 accctgagga gcggcccact tttgagtacc tgcaggcctt cctggaggac tacttcacct
     1621 cgacagagcc ccagtaccag cctggagaga acctacggga tccaccggtg gctaccatgg
     1681 tgagcaaggg cgaggaggtc atcaaggagt tcatgcgctt caaggagcac atggagggct
     1741 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1801 cccagaccgc caggctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1861 tgtcccctca gatcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1921 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1981 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2041 aggtgaaggt gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2101 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2161 tgaagatgag gctgaggctg aaggacggcg gccactacga cgccgaggtc aagaccacct
     2221 acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca
     2281 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc
     2341 actccaccgg cgcctaagcg gccgcgactc tagatcataa tcagccatac cacattga
//
