LOCUS       sequence-86867-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..40
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             269..949
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     670..689
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     864..887
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
       61 ccgctagcgc taccggactc agatctggta cccctttgca gcttccagtt gtcaggaaga
      121 gaaggctccc atggatcctg tggtggtgct ggggctctgt ctctcctgtt tgcttctcct
      181 ttcactctgg aaacagagct atgggggagg gaagcttcga attctgcagt cgacggtacc
      241 gcgggcccgg gatccaccgg tggctaccat ggtgagcaag ggcgaggagg tcatcaagga
      301 gttcatgcgc ttcaaggagc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      361 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaggctga aggtgaccaa
      421 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagatcatgt acggctccaa
      481 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      541 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      601 ctcctccctg caggacggcg agttcatcta caaggtgaag gtgcgcggca ccaacttccc
      661 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      721 gtaccccgag gacggcgccc tgaagggcga gatgaagatg aggctgaggc tgaaggacgg
      781 cggccactac gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc
      841 cggcgcctac aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat
      901 cgtggaacag tacgagcgcg ccgagggccg ccactccacc ggcgcctaag cggccgcgac
      961 ttcttagatc ataatcagcc ataccacaat tgga
//
